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PMID:19098896

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Citation

Jovanovic-Talisman, T, Tetenbaum-Novatt, J, McKenney, AS, Zilman, A, Peters, R, Rout, MP and Chait, BT (2009) Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Nature 457:1023-7

Abstract

Nuclear pore complexes (NPCs) act as effective and robust gateways between the nucleus and the cytoplasm, selecting for the passage of particular macromolecules across the nuclear envelope. NPCs comprise an elaborate scaffold that defines a approximately 30 nm diameter passageway connecting the nucleus and the cytoplasm. This scaffold anchors proteins termed 'phenylalanine-glycine' (FG)-nucleoporins, the natively disordered domains of which line the passageway and extend into its lumen. Passive diffusion through this lined passageway is hindered in a size-dependent manner. However, transport factors and their cargo-bound complexes overcome this restriction by transient binding to the FG-nucleoporins. To test whether a simple passageway and a lining of transport-factor-binding FG-nucleoporins are sufficient for selective transport, we designed a functionalized membrane that incorporates just these two elements. Here we demonstrate that this membrane functions as a nanoselective filter, efficiently passing transport factors and transport-factor-cargo complexes that specifically bind FG-nucleoporins, while significantly inhibiting the passage of proteins that do not. This inhibition is greatly enhanced when transport factor is present. Determinants of selectivity include the passageway diameter, the length of the nanopore region coated with FG-nucleoporins, the binding strength to FG-nucleoporins, and the antagonistic effect of transport factors on the passage of proteins that do not specifically bind FG-nucleoporins. We show that this artificial system faithfully reproduces key features of trafficking through the NPC, including transport-factor-mediated cargo import.

Links

PubMed PMC2764719 Online version:10.1038/nature07600

Keywords

Active Transport, Cell Nucleus; Biomimetic Materials/chemistry; Biomimetic Materials/metabolism; Diffusion; Glycine/metabolism; Gold/chemistry; Karyopherins/metabolism; Membranes, Artificial; Models, Biological; Nanostructures/chemistry; Nuclear Pore/metabolism; Nuclear Pore Complex Proteins/metabolism; Nucleocytoplasmic Transport Proteins/metabolism; Phenylalanine/metabolism; Substrate Specificity

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:NSP1

enables

GO:0017056: structural constituent of nuclear pore

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:NSP1

enables

GO:0017056: structural constituent of nuclear pore

ECO:0000353: physical interaction evidence used in manual assertion

SGD:S000000811
SGD:S000004339

F

Seeded From UniProt

complete

YEAST:NSP1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q06142

F

Seeded From UniProt

complete

HUMAN:NTF2

enables

GO:0042802: identical protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P61970

F

Seeded From UniProt

complete

HUMAN:NTF2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P62826

F

Seeded From UniProt

complete

HUMAN:RAN

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P61970

F

Seeded From UniProt

complete

YEAST:NU100

enables

GO:0017056: structural constituent of nuclear pore

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:NU100

enables

GO:0017056: structural constituent of nuclear pore

ECO:0000353: physical interaction evidence used in manual assertion

SGD:S000004339

F

Seeded From UniProt

complete

YEAST:NU100

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q06142

F

Seeded From UniProt

complete

YEAST:IMA1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q06142

F

Seeded From UniProt

complete

YEAST:IMB1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P14907

F

Seeded From UniProt

complete

YEAST:IMB1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q02629

F

Seeded From UniProt

complete

YEAST:IMB1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q02821

F

Seeded From UniProt

complete


See also

References

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