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PMID:19088087

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Citation

Gaziova, I and Bhat, KM (2009) Ancestry-independent fate specification and plasticity in the developmental timing of a typical Drosophila neuronal lineage. Development 136:263-74

Abstract

In the Drosophila CNS, combinatorial, interdependent, sequential genetic programs in neuroectodermal (NE) cells, prior to the formation of neuroblasts (NBs), determine the initial identity of NBs. Temporal factors are then sequentially expressed to change the temporal identity. It is unclear at what levels this positional and temporal information integrates to determine progeny cell identity. One idea is that this is a top-down linear process: the identity of a NB determines the identity of its daughter, the ganglion mother cell (GMC), the asymmetric division of the GMC and the fate specification of daughter cells of the GMC. Our results with midline (mid), which encodes a T-box protein, in a typical lineage, NB4-2-->GMC-1-->RP2/sib, suggest that at least part of the process operates in GMCs. That is, a GMC or a neuronal identity need not be determined at the NB or NE level. This is demonstrated by showing that Mid is expressed in a row 5 GMC (M-GMC), but not in its parent NB or NE cell. In mid mutants, M-GMC changes into GMC-1 and generates an RP2 and a sib without affecting the expression of key genes at the NE/NB levels. Expression of Mid in the M-GMC in mid mutants rescues the fate change, indicating that Mid specifies neurons at the GMC level. Moreover, we found a significant plasticity in the temporal window in which a neuronal lineage can develop. Although the extra GMC-1 in mid mutants is born approximately 2 hours later than the bona fide GMC-1, it follows the same developmental pattern as the bona fide GMC-1. Thus, a GMC identity can be independent of parental identity and GMC formation and elaboration need not be strictly time-bound.

Links

PubMed PMC2685969 Online version:10.1242/dev.027854

Keywords

Animals; Animals, Genetically Modified; Base Sequence; Central Nervous System/cytology; Central Nervous System/embryology; Central Nervous System/metabolism; DNA/genetics; Drosophila/cytology; Drosophila/embryology; Drosophila/genetics; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Epistasis, Genetic; Genes, Insect; Homeodomain Proteins/genetics; Homeodomain Proteins/metabolism; Molecular Sequence Data; Mutation; Neurogenesis/genetics; Neurons/cytology; Nuclear Proteins/genetics; Phenotype; Promoter Regions, Genetic; T-Box Domain Proteins/genetics; Time Factors; Trans-Activators/genetics; Transcription Factors/metabolism; Wnt1 Protein/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:O62535

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:WNTG

involved_in

GO:0014019: neuroblast development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:NUMB

involved_in

GO:0009786: regulation of asymmetric cell division

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:NUMB

involved_in

GO:0051960: regulation of nervous system development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q24367

involved_in

GO:0009786: regulation of asymmetric cell division

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q24367

involved_in

GO:0051960: regulation of nervous system development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q5BIH3

involved_in

GO:0009786: regulation of asymmetric cell division

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q5BIH3

involved_in

GO:0051960: regulation of nervous system development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8SYY8

involved_in

GO:0007402: ganglion mother cell fate determination

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8SYY8

involved_in

GO:0051960: regulation of nervous system development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8SYY8

involved_in

GO:0048665: neuron fate specification

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8SYY8

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q94543

involved_in

GO:0009786: regulation of asymmetric cell division

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q94543

involved_in

GO:0051960: regulation of nervous system development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:H15

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VMR2

involved_in

GO:0007402: ganglion mother cell fate determination

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VMR2

involved_in

GO:0051960: regulation of nervous system development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VMR2

involved_in

GO:0048665: neuron fate specification

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VMR2

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9W2R4

involved_in

GO:0009786: regulation of asymmetric cell division

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9W2R4

involved_in

GO:0051960: regulation of nervous system development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9W3F4

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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