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PMID:19074766

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Citation

Ithychanda, SS, Das, M, Ma, YQ, Ding, K, Wang, X, Gupta, S, Wu, C, Plow, EF and Qin, J (2009) Migfilin, a molecular switch in regulation of integrin activation. J. Biol. Chem. 284:4713-22

Abstract

The linkage of heterodimeric (alpha/beta) integrin receptors with their extracellular matrix ligands and intracellular actin cytoskeleton is a fundamental step for controlling cell adhesion and migration. Binding of the actin-linking protein, talin, to integrin beta cytoplasmic tails (CTs) induces high affinity ligand binding (integrin activation), whereas binding of another actin-linking protein, filamin, to the integrin beta CTs negatively regulates this process by blocking the talin-integrin interaction. Here we show structurally that migfilin, a novel cytoskeletal adaptor highly enriched in the integrin adhesion sites, strongly interacts with the same region in filamin where integrin beta CTs bind. We further demonstrate that the migfilin interaction dissociates filamin from integrin and promotes the talin/integrin binding and integrin activation. Migfilin thus acts as a molecular switch to disconnect filamin from integrin for regulating integrin activation and dynamics of extracellular matrix-actin linkage.

Links

PubMed PMC2640964 Online version:10.1074/jbc.M807719200

Keywords

Actins/genetics; Actins/metabolism; Animals; CHO Cells; Cell Adhesion/physiology; Cell Adhesion Molecules/genetics; Cell Adhesion Molecules/metabolism; Cell Movement/physiology; Contractile Proteins/genetics; Contractile Proteins/metabolism; Cricetinae; Cricetulus; Cytoskeletal Proteins/genetics; Cytoskeletal Proteins/metabolism; Cytoskeleton/genetics; Cytoskeleton/metabolism; Extracellular Matrix/genetics; Extracellular Matrix/metabolism; Filamins; Humans; Integrin alpha Chains/genetics; Integrin alpha Chains/metabolism; Integrin beta Chains/genetics; Integrin beta Chains/metabolism; Microfilament Proteins/genetics; Microfilament Proteins/metabolism; Talin/genetics; Talin/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:FBLI1

involved_in

GO:0098609: cell-cell adhesion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:FBLI1

involved_in

GO:0033623: regulation of integrin activation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:FBLI1

enables

GO:0031005: filamin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

See also

References

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