GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:19073882

From GONUTS
Jump to: navigation, search
Citation

Songer, JA and Munson, M (2009) Sec6p anchors the assembled exocyst complex at sites of secretion. Mol. Biol. Cell 20:973-82

Abstract

The exocyst is an essential protein complex required for targeting and fusion of secretory vesicles to sites of exocytosis at the plasma membrane. To study the function of the exocyst complex, we performed a structure-based mutational analysis of the Saccharomyces cerevisiae exocyst subunit Sec6p. Two "patches" of highly conserved residues are present on the surface of Sec6p; mutation of either patch does not compromise protein stability. Nevertheless, replacement of SEC6 with the patch mutants results in severe temperature-sensitive growth and secretion defects. At nonpermissive conditions, although trafficking of secretory vesicles to the plasma membrane is unimpaired, none of the exocyst subunits are polarized. This is consistent with data from other exocyst temperature-sensitive mutants, which disrupt the integrity of the complex. Surprisingly, however, these patch mutations result in mislocalized exocyst complexes that remain intact. Our results indicate that assembly and polarization of the exocyst are functionally separable events, and that Sec6p is required to anchor exocyst complexes at sites of secretion.

Links

PubMed PMC2633393 Online version:10.1091/mbc.E08-09-0968

Keywords

Alleles; Amino Acid Sequence; Amino Acids/metabolism; Carrier Proteins/chemistry; Carrier Proteins/metabolism; Conserved Sequence; Exosomes/metabolism; Models, Molecular; Molecular Sequence Data; Mutant Proteins/metabolism; Mutation/genetics; Protein Binding; Protein Subunits/metabolism; Protein Transport; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/metabolism; Vesicular Transport Proteins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:SEC6

located_in

GO:0005935: cellular bud neck

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:SEC6

located_in

GO:0005934: cellular bud tip

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:SEC6

involved_in

GO:0051601: exocyst localization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.