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PMID:19060108

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Citation

Smertenko, AP, Kaloriti, D, Chang, HY, Fiserova, J, Opatrny, Z and Hussey, PJ (2008) The C-terminal variable region specifies the dynamic properties of Arabidopsis microtubule-associated protein MAP65 isotypes. Plant Cell 20:3346-58

Abstract

The microtubule-associated protein, MAP65, is a member of a family of divergent microtubule-associated proteins from different organisms generally involved in maintaining the integrity of the central spindle in mitosis. The dicotyledon Arabidopsis thaliana and the monocotyledon rice (Oryza sativa) genomes contain 9 and 11 MAP65 genes, respectively. In this work, we show that the majority of these proteins fall into five phylogenetic clades, with the greatest variation between clades being in the C-terminal random coil domain. At least one Arabidopsis and one rice isotype is within each clade, indicating a functional specification for the C terminus. In At MAP65-1, the C-terminal domain is a microtubule binding region (MTB2) harboring the phosphorylation sites that control its activity. The At MAP65 isotypes show differential localization to microtubule arrays and promote microtubule polymerization with variable efficiency in a MTB2-dependent manner. In vivo studies demonstrate that the dynamics of the association and dissociation of different MAP65 isotypes with microtubules can vary up to 10-fold and that this correlates with their ability to promote microtubule polymerization. Our data demonstrate that the C-terminal variable region, MTB2, determines the dynamic properties of individual isotypes and suggest that slower turnover is conditional for more efficient microtubule polymerization.

Links

PubMed PMC2630438 Online version:10.1105/tpc.108.063362

Keywords

Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Immunoblotting; Microscopy, Confocal; Microtubule-Associated Proteins/chemistry; Microtubule-Associated Proteins/genetics; Microtubule-Associated Proteins/metabolism; Microtubules/metabolism; Plant Proteins/chemistry; Plant Proteins/genetics; Plant Proteins/metabolism; Protein Binding/genetics; Protein Isoforms/chemistry; Protein Isoforms/genetics; Protein Isoforms/metabolism; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:F4K3E4

part_of

GO:0009524: phragmoplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:F4K3E4

part_of

GO:0009574: preprophase band

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:F4K3E4

part_of

GO:0055028: cortical microtubule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:F4K3E4

enables

GO:0008017: microtubule binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:F4K3E4

involved_in

GO:0046785: microtubule polymerization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:MA659

NOT|part_of

GO:0005874: microtubule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:MA652

part_of

GO:1990023: mitotic spindle midzone

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:MA652

part_of

GO:0009524: phragmoplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:MA652

part_of

GO:0009574: preprophase band

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:MA652

part_of

GO:0055028: cortical microtubule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:MA658

NOT|part_of

GO:0005874: microtubule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:MA653

enables

GO:0008017: microtubule binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:MA653

part_of

GO:0009524: phragmoplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:MA653

part_of

GO:0009574: preprophase band

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:MA653

part_of

GO:0055028: cortical microtubule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:MA653

involved_in

GO:0046785: microtubule polymerization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:MA656

part_of

GO:0009524: phragmoplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:MA656

part_of

GO:0009574: preprophase band

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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