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PMID:19060108
| Citation |
Smertenko, AP, Kaloriti, D, Chang, HY, Fiserova, J, Opatrny, Z and Hussey, PJ (2008) The C-terminal variable region specifies the dynamic properties of Arabidopsis microtubule-associated protein MAP65 isotypes. Plant Cell 20:3346-58 |
|---|---|
| Abstract |
The microtubule-associated protein, MAP65, is a member of a family of divergent microtubule-associated proteins from different organisms generally involved in maintaining the integrity of the central spindle in mitosis. The dicotyledon Arabidopsis thaliana and the monocotyledon rice (Oryza sativa) genomes contain 9 and 11 MAP65 genes, respectively. In this work, we show that the majority of these proteins fall into five phylogenetic clades, with the greatest variation between clades being in the C-terminal random coil domain. At least one Arabidopsis and one rice isotype is within each clade, indicating a functional specification for the C terminus. In At MAP65-1, the C-terminal domain is a microtubule binding region (MTB2) harboring the phosphorylation sites that control its activity. The At MAP65 isotypes show differential localization to microtubule arrays and promote microtubule polymerization with variable efficiency in a MTB2-dependent manner. In vivo studies demonstrate that the dynamics of the association and dissociation of different MAP65 isotypes with microtubules can vary up to 10-fold and that this correlates with their ability to promote microtubule polymerization. Our data demonstrate that the C-terminal variable region, MTB2, determines the dynamic properties of individual isotypes and suggest that slower turnover is conditional for more efficient microtubule polymerization. |
| Links |
PubMed PMC2630438 Online version:10.1105/tpc.108.063362 |
| Keywords |
Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Immunoblotting; Microscopy, Confocal; Microtubule-Associated Proteins/chemistry; Microtubule-Associated Proteins/genetics; Microtubule-Associated Proteins/metabolism; Microtubules/metabolism; Plant Proteins/chemistry; Plant Proteins/genetics; Plant Proteins/metabolism; Protein Binding/genetics; Protein Isoforms/chemistry; Protein Isoforms/genetics; Protein Isoforms/metabolism; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
ARATH:F4K3E4 |
part_of |
GO:0009524: phragmoplast |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
ARATH:F4K3E4 |
part_of |
GO:0009574: preprophase band |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
ARATH:F4K3E4 |
part_of |
GO:0055028: cortical microtubule |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
ARATH:F4K3E4 |
enables |
GO:0008017: microtubule binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
ARATH:F4K3E4 |
involved_in |
GO:0046785: microtubule polymerization |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
ARATH:MA659 |
NOT|part_of |
GO:0005874: microtubule |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
ARATH:MA652 |
part_of |
GO:1990023: mitotic spindle midzone |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
ARATH:MA652 |
part_of |
GO:0009524: phragmoplast |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
ARATH:MA652 |
part_of |
GO:0009574: preprophase band |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
ARATH:MA652 |
part_of |
GO:0055028: cortical microtubule |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
ARATH:MA658 |
NOT|part_of |
GO:0005874: microtubule |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
ARATH:MA653 |
enables |
GO:0008017: microtubule binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
ARATH:MA653 |
part_of |
GO:0009524: phragmoplast |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
ARATH:MA653 |
part_of |
GO:0009574: preprophase band |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
ARATH:MA653 |
part_of |
GO:0055028: cortical microtubule |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
ARATH:MA653 |
involved_in |
GO:0046785: microtubule polymerization |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
ARATH:MA656 |
part_of |
GO:0009524: phragmoplast |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
ARATH:MA656 |
part_of |
GO:0009574: preprophase band |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
See also
References
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