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PMID:19050011
| Citation |
Green, LM, Wagner, KJ, Campbell, HA, Addison, K and Roberts, SG (2009) Dynamic interaction between WT1 and BASP1 in transcriptional regulation during differentiation. Nucleic Acids Res. 37:431-40 |
|---|---|
| Abstract |
The Wilms' tumour suppressor protein WT1 plays a central role in the development of the kidney and also other organs. WT1 can act as a transcription factor with highly context-specific activator and repressor functions. We previously identified Brain Acid Soluble Protein 1 (BASP1) as a transcriptional cosuppressor that can block the transcriptional activation function of WT1. WT1 and BASP1 are co-expressed during nephrogenesis and both proteins ultimately become restricted to the podocyte cells of the adult kidney. Here, we have analysed the WT1/BASP1 complex in a podocyte precursor cell line that can be induced to differentiate. Chromatin immunoprecipitation revealed that WT1 and BASP1 occupy the promoters of the Bak, c-myc and podocalyxin genes in podocyte precursor cells. During differentiation-dependent upregulation of podocalyxin expression BASP1 occupancy of the podocalyxin promoter is reduced compared to that of WT1. In contrast, the repressive WT1/BASP1 occupancy of the c-myc and Bak promoters is maintained and these genes are downregulated during the differentiation process. We provide evidence that the regulation of BASP1 promoter occupancy involves the sumoylation of BASP1. Our results reveal a dynamic cooperation between WT1 and BASP1 in the regulation of gene expression during differentiation. |
| Links |
PubMed PMC2632906 Online version:10.1093/nar/gkn955 |
| Keywords |
Animals; Calmodulin-Binding Proteins/analysis; Calmodulin-Binding Proteins/metabolism; Cell Differentiation; Cell Line; Cell Nucleus/chemistry; Cytoskeletal Proteins/analysis; Cytoskeletal Proteins/metabolism; Gene Expression Regulation; Humans; Nerve Tissue Proteins/analysis; Nerve Tissue Proteins/metabolism; Podocytes/cytology; Podocytes/metabolism; Promoter Regions, Genetic; Repressor Proteins/metabolism; Stem Cells/metabolism; Trans-Activators/metabolism; Transcription, Genetic; WT1 Proteins/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:Q91XV3 |
F |
Seeded From UniProt |
complete | ||
|
located_in |
GO:0005634: nucleus |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
HUMAN:SUMO3 |
located_in |
GO:0016605: PML body |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | |
|
HUMAN:SUMO3 |
involved_in |
GO:0016925: protein sumoylation |
ECO:0000314: direct assay evidence used in manual assertion |
P |
|
Seeded From UniProt |
complete | |
|
HUMAN:SUMO3 |
involved_in |
GO:1900180: regulation of protein localization to nucleus |
ECO:0000314: direct assay evidence used in manual assertion |
P |
|
Seeded From UniProt |
complete | |
|
HUMAN:BASP1 |
located_in |
GO:0016605: PML body |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:BASP1 |
located_in |
GO:0016363: nuclear matrix |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:BASP1 |
involved_in |
GO:0045892: negative regulation of transcription, DNA-templated |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
MOUSE:BASP1 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P22561 |
F |
Seeded From UniProt |
complete | |
|
MOUSE:BASP1 |
located_in |
GO:0000785: chromatin |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
MOUSE:BASP1 |
enables |
GO:0000976: transcription regulatory region sequence-specific DNA binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
MOUSE:BASP1 |
located_in |
GO:0016607: nuclear speck |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
MOUSE:BASP1 |
involved_in |
GO:0072112: glomerular visceral epithelial cell differentiation |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0045892: negative regulation of transcription, DNA-templated |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634: nucleus |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0072112: glomerular visceral epithelial cell differentiation |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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