GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:18973764

From GONUTS
Jump to: navigation, search
Citation

Ko, SI, Park, JH, Park, MJ, Kim, J, Kang, LW and Han, YS (2008) Human ribosomal protein S3 (hRpS3) interacts with uracil-DNA glycosylase (hUNG) and stimulates its glycosylase activity. Mutat. Res. 648:54-64

Abstract

Human ribosomal protein S3 (hRpS3) is a small ribosomal subunit showing apurinic/apyrimidinic (AP) lyase activity and has been suggested to play a role in the cellular DNA-damage response pathway. However, the functional interactions between hRpS3 and other base excision repair (BER) DNA glycosylases have not been reported. We identified, for the first time, the interaction between hRpS3 and human uracil-DNA glycosylase (hUNG) and investigated the functional consequences of this interaction. hRpS3 was shown to interact with hUNG in co-immunoprecipitation assay using transiently transfected HEK293 cells and GST pull-down assay using microbial expression systems. In an assay using a 5'-end-radiolabeled 39-mer oligonucleotide duplex containing a U/G mismatch, hRpS3 dramatically stimulated the uracil-excision activity of hUNG, whereas hRpS3 alone had no cleavage activity. Pre-incubation of hRpS3 with the U/G mismatch containing DNA duplex also increased the hUNG uracil-excision activity; however, hRpS3 did not increase the DNA binding activity of hUNG in a trapping assay of hUNG and the U/G mismatch containing DNA duplex using UV cross-linking. hRpS3 has been suggested to stimulate the uracil-excision activity of hUNG by enhancing its dissociation from AP sites and increasing its turn-over rate. The disruption of hRpS3 by small-interfering RNA (siRNA-hRpS3) transfection reduced the uracil-excision activity preserved in cell extracts, whereas the supplement of purified hRpS3 retained uracil-excision activity. These results strongly suggest that hRpS3 may be involved in the uracil-excision pathway, probably by participating in the DNA repair mechanism to remove uracil generated by the deamination of cytosine in DNA, and by preventing C/G-->T/A transition mutations.

Links

PubMed Online version:10.1016/j.mrfmmm.2008.09.013

Keywords

Base Pair Mismatch/physiology; Cells, Cultured; DNA/metabolism; DNA Repair/physiology; DNA-(Apurinic or Apyrimidinic Site) Lyase/metabolism; Enzyme Activation; Gene Knockdown Techniques; Humans; Point Mutation/physiology; Protein Binding; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Ribosomal Proteins/metabolism; Ribosomal Proteins/physiology; Transfection; Uracil/metabolism; Uracil-DNA Glycosidase/genetics; Uracil-DNA Glycosidase/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:UNG

enables

GO:0003684: damaged DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:UNG

involved_in

GO:0097510: base-excision repair, AP site formation via deaminated base removal

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:UNG

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P23396

F

Seeded From UniProt

complete

HUMAN:UNG

enables

GO:0043024: ribosomal small subunit binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P23396

F

Seeded From UniProt

complete

HUMAN:RS3

involved_in

GO:0032079: positive regulation of endodeoxyribonuclease activity

ECO:0000314: direct assay evidence used in manual assertion

P

  • has_input:(UniProtKB:P27695)

Seeded From UniProt

complete

HUMAN:RS3

involved_in

GO:1905053: positive regulation of base-excision repair

ECO:0000314: direct assay evidence used in manual assertion

P

  • has_input:(UniProtKB:P13051)

Seeded From UniProt

complete

HUMAN:RS3

enables

GO:0019899: enzyme binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P13051

F

Seeded From UniProt

complete

HUMAN:APEX1

enables

GO:0003906: DNA-(apurinic or apyrimidinic site) endonuclease activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:APEX1

enables

GO:0008081: phosphoric diester hydrolase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

Notes

See also

References

See Help:References for how to manage references in GONUTS.