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PMID:18950725

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Citation

Koskinen, J, Karlsson, J and Olsson, PE (2009) Sox9a regulation of ff1a in zebrafish (Danio rerio) suggests an involvement of ff1a in cartilage development. Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. 153:39-43

Abstract

The NR5A family of orphan nuclear receptors has been implicated in development of the vertebrate embryo, but their exact role remains largely unknown. To evaluate the regulation and developmental role for ff1a (NR5A2) in zebrafish (Danio rerio), we performed morpholino knockdown to block translation of the ff1a gene and the upstream located sox9a gene during embryogenesis. Using a newly developed antibody against Ff1a we could show that the ff1a morpholinos were functional and that a reduction in the expression of Ff1a correlated to altered phenotypes. The role of Sox9a in ff1a gene regulation and function was studied using sox9a morpholinos. Knock-down of sox9a resulted in abolished ff1a signals in the somites, mandibular arches and pharyngeal arches, while the pectoral fin signal remained. The reduction in Ff1a levels correlated to truncated tails and cranio-facial malformation. As Sox9a is involved in chondrocyte development we analysed for cartilage formation and found that blocking translation of either sox9a or ff1a also blocked cartilage formation. In light of the results, the present study suggests a novel function of ff1a in chondrocyte development.

Links

PubMed Online version:10.1016/j.cbpa.2008.10.003

Keywords

Alcian Blue; Animals; Blotting, Western; Cartilage/embryology; Cartilage/metabolism; Embryo, Nonmammalian/metabolism; Gene Expression Regulation, Developmental; Gonads/metabolism; In Situ Hybridization; Liver/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; Receptors, Cytoplasmic and Nuclear/genetics; Receptors, Cytoplasmic and Nuclear/metabolism; SOX9 Transcription Factor/genetics; SOX9 Transcription Factor/metabolism; Zebrafish/embryology; Zebrafish/genetics; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:F1Q8I8

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-070412-2

P

Seeded From UniProt

complete

DANRE:F1Q8I8

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-070412-1

P

Seeded From UniProt

complete

DANRE:O42186

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100428-2

P

Seeded From UniProt

complete

DANRE:O42186

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-051025-2

P

Seeded From UniProt

complete

DANRE:O42557

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100428-2

P

Seeded From UniProt

complete

DANRE:O42557

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-051025-2

P

Seeded From UniProt

complete

DANRE:Q567L2

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-070412-2

P

Seeded From UniProt

complete

DANRE:Q567L2

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-070412-1

P

Seeded From UniProt

complete

DANRE:Q8AY39

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-070412-2

P

Seeded From UniProt

complete

DANRE:Q8AY39

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-070412-1

P

Seeded From UniProt

complete

DANRE:Q90YL6

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100428-2

P

Seeded From UniProt

complete

DANRE:Q90YL6

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-051025-2

P

Seeded From UniProt

complete

DANRE:Q9DFB4

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100428-2

P

Seeded From UniProt

complete

DANRE:Q9DFB4

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-051025-2

P

Seeded From UniProt

complete

DANRE:Q9DFB5

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100428-2

P

Seeded From UniProt

complete

DANRE:Q9DFB5

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-051025-2

P

Seeded From UniProt

complete

DANRE:Q9DFH2

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-070412-2

P

Seeded From UniProt

complete

DANRE:Q9DFH2

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-070412-1

P

Seeded From UniProt

complete


See also

References

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