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PMID:18936963

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Citation

Hong, JK and Hwang, BK (2009) The promoter of the pepper pathogen-induced membrane protein gene CaPIMP1 mediates environmental stress responses in plants. Planta 229:249-59

Abstract

The promoter of the pepper pathogen-induced membrane protein gene CaPIMP1 was analyzed by an Agrobacterium-mediated transient expression assay in tobacco leaves. Several stress-related cis-acting elements (GT-1, W-box and ABRE) are located within the CaPIMP1 promoter. In tobacco leaf tissues transiently transformed with a CaPIMP1 promoter-beta-glucuronidase (GUS) gene fusion, serially 5'-deleted CaPIMP1 promoters were differentially activated by Pseudomonas syringae pv. tabaci, ethylene, methyl jasmonate, abscisic acid, and nitric oxide. The -1,193 bp region of the CaPIMP1 gene promoter sequence exhibited full promoter activity. The -417- and -593 bp promoter regions were sufficient for GUS gene activation by ethylene and methyl jasmonate treatments, respectively. However, CaPIMP1 promoter sequences longer than -793 bp were required for promoter activation by abscisic acid and sodium nitroprusside treatments. CaPIMP1 expression was activated in pepper leaves by treatment with ethylene, methyl jasmonate, abscisic acid, beta-amino-n-butyric acid, NaCl, mechanical wounding, and low temperature, but not with salicylic acid. Overexpression of CaPIMP1 in Arabidopsis conferred hypersensitivity to mannitol, NaCl, and ABA during seed germination but not during seedling development. In contrast, transgenic plants overexpressing CaPIMP1 exhibited enhanced tolerance to oxidative stress induced by methyl viologen during germination and early seedling stages. These results suggest that CaPIMP1 expression may alter responsiveness to environmental stress, as well as to pathogen infection.

Links

PubMed Online version:10.1007/s00425-008-0824-z

Keywords

Abscisic Acid/pharmacology; Acetates/pharmacology; Adaptation, Physiological/drug effects; Arabidopsis/drug effects; Base Sequence; Capsicum/drug effects; Capsicum/genetics; Cyclopentanes/pharmacology; Environment; Ethylenes/pharmacology; Gene Expression Regulation, Plant/drug effects; Genes, Plant; Membrane Proteins/genetics; Molecular Sequence Data; Osmotic Pressure/drug effects; Oxylipins/pharmacology; Plant Leaves/drug effects; Plant Leaves/genetics; Plant Proteins/genetics; Plant Proteins/metabolism; Promoter Regions, Genetic/genetics; Pseudomonas syringae/drug effects; Pseudomonas syringae/physiology; Sequence Analysis, DNA; Signal Transduction/drug effects; Stress, Physiological/drug effects; Tobacco/drug effects; Tobacco/microbiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAPAN:PIMP1

involved_in

GO:0009409: response to cold

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CAPAN:PIMP1

involved_in

GO:0009611: response to wounding

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CAPAN:PIMP1

involved_in

GO:0009617: response to bacterium

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CAPAN:PIMP1

involved_in

GO:0009651: response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CAPAN:PIMP1

involved_in

GO:0009723: response to ethylene

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CAPAN:PIMP1

involved_in

GO:0009737: response to abscisic acid

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CAPAN:PIMP1

NOT|involved_in

GO:0009751: response to salicylic acid

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CAPAN:PIMP1

involved_in

GO:0009753: response to jasmonic acid

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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