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PMID:18936075

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Citation

Arthur, P, Taggart, MJ, Zielnik, B, Wong, S and Mitchell, BF (2008) Relationship between gene expression and function of uterotonic systems in the rat during gestation, uterine activation and both term and preterm labour. J. Physiol. (Lond.) 586:6063-76

Abstract

We have documented gestation- and labour- (preterm and term) dependent changes in expression of genes encoding contraction associated proteins in the rat uterus and correlated these changes with various parameters of uterine contractility. The data demonstrate increased expression of contractile agonist systems concurrent with decreased expression of relaxant systems after gestational day 20. Significant increases in expression of oxytocin (OT), its receptor (OTR), prostaglandin (PG) H synthase isoform 1 (PGHS-1) and PGF(2alpha) receptor (FP) occurred first, followed by increases in PGHS-2, connexin-43, endothelin-1 (ET-1) and the ET-1 receptor isoform ET(A). Expression of OTR and FP was significantly reduced during mid-gestation compared to non-pregnant animals. Expression of inducible nitric oxide synthase (iNOS) increased significantly during pregnancy then decreased concurrently with the increase in OTR and FP. Functional changes in uterine contractility accompany changes in gene expression. OT was the most potent contractile stimulant. Sensitivity of uterine strips to OT was reduced in early and mid-pregnancy then increased at uterine activation. Progesterone antagonist-induced preterm labour caused changes similar to those at normal term. Comparison of mRNA transcripts in separated endometrium and myometrium suggested that the endometrium is an important regulator of myometrial contractility, analogous to the relationship between endothelium and vascular smooth muscle. This novel combination of functional and genetic expression analyses provides new insight into the physiology of parturition.

Links

PubMed PMC2655414 Online version:10.1113/jphysiol.2008.164004

Keywords

Animals; Connexin 43/genetics; Cyclooxygenase 1/genetics; Cyclooxygenase 2/genetics; Dinoprost/pharmacology; Endothelin-1/genetics; Female; Gene Expression/drug effects; Gene Expression Profiling; Gestational Age; Male; Mifepristone/pharmacology; Myography; Nitric Oxide Synthase Type II/genetics; Oxytocin/genetics; Pregnancy; Rats; Rats, Sprague-Dawley; Receptors, Endothelin/genetics; Receptors, Oxytocin/genetics; Reverse Transcriptase Polymerase Chain Reaction; Time Factors; Uterine Contraction/drug effects; Uterus/drug effects; Uterus/metabolism; Uterus/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:OXYR

involved_in

GO:0060137: maternal process involved in parturition

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:NEU1

involved_in

GO:0007565: female pregnancy

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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