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PMID:18927441

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Citation

Lam, EY, Chau, JY, Kalev-Zylinska, ML, Fountaine, TM, Mead, RS, Hall, CJ, Crosier, PS, Crosier, KE and Flores, MV (2009) Zebrafish runx1 promoter-EGFP transgenics mark discrete sites of definitive blood progenitors. Blood 113:1241-9

Abstract

The transcription factor Runx1 is essential for the development of definitive hematopoietic stem cells (HSCs) during vertebrate embryogenesis and is transcribed from 2 promoters, P1 and P2, generating 2 major Runx1 isoforms. We have created 2 stable runx1 promoter zebrafish-transgenic lines that provide insight into the roles of the P1 and P2 isoforms during the establishment of definitive hematopoiesis. The Tg(runx1P1:EGFP) line displays fluorescence in the posterior blood island, where definitive erythromyeloid progenitors develop. The Tg(runx1P2:EGFP) line marks definitive HSCs in the aorta-gonad-mesonephros, with enhanced green fluorescent protein-labeled cells later populating the pronephros and thymus. This suggests that a function of runx1 promoter switching is associated with the establishment of discrete definitive blood progenitor compartments. These runx1 promoter-transgenic lines are novel tools for the study of Runx1 regulation and function in normal and malignant hematopoiesis. The ability to visualize and isolate fluorescently labeled HSCs should contribute to further elucidating the complex regulation of HSC development.

Links

PubMed Online version:10.1182/blood-2008-04-149898

Keywords

Animals; Animals, Genetically Modified; Blotting, Southern; Cell Lineage; Core Binding Factor Alpha 2 Subunit/physiology; Embryo, Nonmammalian/cytology; Embryo, Nonmammalian/metabolism; Erythroid Precursor Cells/cytology; Gene Expression Regulation, Developmental; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Hematopoiesis; Immunoenzyme Techniques; In Situ Hybridization; Mesonephros/cytology; Mesonephros/embryology; Promoter Regions, Genetic/genetics; Protein Isoforms; Zebrafish; Zebrafish Proteins/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:Q9DGB8

involved_in

GO:0060216: definitive hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100813-4

P

Seeded From UniProt

complete


See also

References

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