PMID:18852242

Citation	Garcia, AE, Osapay, G, Tran, PA, Yuan, J and Selsted, ME (2008) Isolation, synthesis, and antimicrobial activities of naturally occurring theta-defensin isoforms from baboon leukocytes. Infect. Immun. 76:5883-91
Abstract	Theta-defensins are macrocyclic antimicrobial peptides that were previously isolated from leukocytes of a single species, the rhesus macaque. We now report the characterization of baboon theta-defensins (BTDs) expressed in bone marrow and peripheral blood leukocytes. Four cDNAs encoding theta-defensin precursors were characterized, allowing for the prediction of 10 theoretical theta-defensins (BTD-1 to BTD-10) produced by binary, head-to-tail splicing of nonapeptides excised from paired precursors. Five of the predicted theta-defensins were purified from baboon leukocytes, and synthetic versions of each were prepared. Anti-theta-defensin antibody localized the peptides in circulating neutrophils and monocytes and in immature and mature myeloid elements in bone marrow. Each of the BTDs possessed antimicrobial activity against bacterial and fungal test organisms in vitro. Peptide activities varied markedly despite a high degree of sequence conservation among the theta-defensins tested. Thus, baboons express numerous theta-defensins which appear to differentially contribute to host defense against diverse pathogens.
Links	PubMed PMC2583559 Online version:10.1128/IAI.01100-08
Keywords	Amino Acid Sequence; Animals; Blotting, Western; Defensins/genetics; Defensins/immunology; Defensins/isolation & purification; Humans; Immunohistochemistry; Leukocytes/immunology; Molecular Sequence Data; Papio/immunology; Protein Isoforms/genetics; Protein Isoforms/immunology; Protein Isoforms/isolation & purification; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
PAPAN:BTDC		GO:0050832:	ECO:0000314:				figure six panel 3 shows c. albicans zone clearance after exposure to BTD-4	complete
PAPAN:BTDC		GO:0050829: defense response to Gram-negative bacterium	ECO:0000314:		P		figure six panel 2 shows e. coli zone clearance after exposure to BTD-4	complete
PAPAN:BTDA		GO:0050832: defense response to fungus	ECO:0000314:		P		figure six panel 3 shows c. albicans zone clearance after exposure to this BTD (subunit 1 of BTD-1, BTD-4, BTD-7, and BTD-3 itself)	complete
PAPAN:BTDA		GO:0050829: defense response to Gram-negative bacterium	ECO:0000314:		P		figure six panel 2 shows e. coli zone clearance after exposure to this BTD (subunit 1 of BTD-1, BTD-4, BTD-7, and BTD-3 itself)	complete
PAPAN:BTDB		GO:0050830: defense response to Gram-positive bacterium	ECO:0000314:		P		figures 3c and 6	complete
PAPAN:BTDB		GO:0050829: defense response to Gram-negative bacterium	ECO:0000314:		P		figure 3c and figure 6	complete
PAPAN:BTDB		GO:0050832: defense response to fungus	ECO:0000314:		P		figure 3c and figure 6	complete
PAPAN:BTDD		GO:0050832: defense response to fungus	ECO:0000314:		P		figure 6	complete
PAPAN:BTDD		GO:0050830: defense response to Gram-positive bacterium	ECO:0000314:		P		figure 6	complete
PAPAN:BTDD		GO:0050829: defense response to Gram-negative bacterium	ECO:0000314:		P		figure 6	complete

References

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