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PMID:18851954

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Citation

Zhai, J, Gao, D, Liu, W, Hong, R, Qin, Y, Ouyang, H, Kong, Y, Wang, Y, Xie, Y and Liu, J (2008) Characterization of a novel isoform of murine interferon regulatory factor 3. Biochem. Biophys. Res. Commun. 377:384-8

Abstract

Interferon (IFN) regulatory factors (IRFs) are a family of transcription mediators involved in the regulation of type I IFN (IFN-alpha/beta) transcription. Among the nine already identified IRFs, IRF-3 is a constitutively and ubiquitously expressed and plays a critical role in the transcriptional activation of type I IFN and IFN-stimulated genes including IFN-alpha1, IFN-beta and RANTES. In the present study, we report the identification of a novel alternatively spliced transcript of murine Irf-3 gene (mIrf-3) designated mIRF-3a. mIRF-3a is ubiquitously present in mouse tissues along with mIRF-3 and could be translocated into the nucleus in uninfected L929 cells. EMSA and reporter assay demonstrated that mIRF-3a binds to ISRE sequences in murine Ifn-beta promoter and represses Ifn-beta promoter activation induced by Newcastle disease virus infection. These results suggest that mIRF-3a may act as a modulator of mIRF-3 functions in vivo.

Links

PubMed Online version:10.1016/j.bbrc.2008.09.147

Keywords

Animals; Cell Line; Cell Nucleus/metabolism; Electrophoretic Mobility Shift Assay; Female; Interferon Regulatory Factor-3/genetics; Interferon Regulatory Factor-3/metabolism; Interferon-beta/genetics; Liver/metabolism; Mice; Mice, Inbred BALB C; Newcastle Disease/genetics; Newcastle Disease/metabolism; Promoter Regions, Genetic; Protein Isoforms/genetics; Protein Isoforms/metabolism; Protein Transport; RNA, Messenger/genetics; RNA, Messenger/metabolism; Tissue Distribution; Transcription, Genetic; Transcriptional Activation

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:C0IXK2

acts_upstream_of_or_within

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

  • has_regulation_target:(MGI:MGI:107657)

Seeded From UniProt

complete

MOUSE:C0IXK2

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:C0IXK2

enables

GO:0000976: transcription regulatory region sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:C0IXK2

acts_upstream_of_or_within

GO:0032688: negative regulation of interferon-beta production

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:C0IXK2

acts_upstream_of_or_within

GO:0050689: negative regulation of defense response to virus by host

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:IRF3

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:Q3U9K6

enables

GO:0000978: RNA polymerase II cis-regulatory region sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:Q3U9K6

enables

GO:0001227: DNA-binding transcription repressor activity, RNA polymerase II-specific

ECO:0000314: direct assay evidence used in manual assertion

F

  • occurs_at:(SO:0001952)

Seeded From UniProt

complete


See also

References

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