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PMID:18832716

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Citation

Engel, DR, Maurer, J, Tittel, AP, Weisheit, C, Cavlar, T, Schumak, B, Limmer, A, van Rooijen, N, Trautwein, C, Tacke, F and Kurts, C (2008) CCR2 mediates homeostatic and inflammatory release of Gr1(high) monocytes from the bone marrow, but is dispensable for bladder infiltration in bacterial urinary tract infection. J. Immunol. 181:5579-86

Abstract

CCR2 is thought to recruit monocytes to sites of infection. Two subpopulations of murine blood monocytes differing in Gr1 and CCR2 expression have been described. The exact role of CCR2 in migration of CCR2(low)Gr1(low) and CCR2(high)Gr1(high) monocytes into nonlymphoid tissue is controversial. In this study, we have addressed this question in a murine model of bacterial urinary tract infection. Only Gr1(high) monocytes were recruited into the infected bladder. CCR2 deficiency reduced their frequency in this organ, indicating a requirement of this chemokine receptor. Importantly, CCR2-deficient mice also showed reduced Gr1(high) monocyte numbers in the blood, but not in the bone marrow (BM), indicating that CCR2 acted at the step of monocyte release into the circulation. The same was found also in noninfected mice, indicating a further involvement of CCR2 in steady-state BM egress. An additional requirement of CCR2 in monocyte recruitment from the blood into the bladder was excluded by tracking particle-labeled endogenous monocytes and by adoptive transfer of BM-derived monocyte subsets. These findings demonstrate that CCR2 governs homeostatic and infection-triggered release of Gr1(high) monocytes from the BM into the blood but is dispensable for recruitment into a nonlymphoid tissue.

Links

PubMed

Keywords

Animals; Antigens, Ly/genetics; Antigens, Ly/immunology; Bacterial Infections/genetics; Bacterial Infections/immunology; Disease Models, Animal; Female; Gene Expression Regulation/genetics; Gene Expression Regulation/immunology; Homeostasis/genetics; Homeostasis/immunology; Humans; Inflammation/genetics; Inflammation/immunology; Male; Mice; Mice, Knockout; Monocytes/immunology; Receptors, CCR2/genetics; Receptors, CCR2/immunology; Urinary Bladder/immunology; Urinary Bladder/microbiology; Urinary Tract Infections/genetics; Urinary Tract Infections/immunology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:CCR2

involved_in

GO:0019725: cellular homeostasis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CCR2

involved_in

GO:0032760: positive regulation of tumor necrosis factor production

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CCR2

involved_in

GO:0090026: positive regulation of monocyte chemotaxis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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