GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:18827010

From GONUTS
Jump to: navigation, search
Citation

Tan, FJ, Husain, M, Manlandro, CM, Koppenol, M, Fire, AZ and Hill, RB (2008) CED-9 and mitochondrial homeostasis in C. elegans muscle. J. Cell. Sci. 121:3373-82

Abstract

Mitochondrial homeostasis reflects a dynamic balance between membrane fission and fusion events thought essential for mitochondrial function. We report here that altered expression of the C. elegans BCL2 homolog CED-9 affects both mitochondrial fission and fusion. Although striated muscle cells lacking CED-9 have no alteration in mitochondrial size or ultrastructure, these cells appear more sensitive to mitochondrial fragmentation. By contrast, increased CED-9 expression in these cells produces highly interconnected mitochondria. This mitochondrial phenotype is partially suppressed by increased expression of the dynamin-related GTPase DRP-1, with suppression dependent on the BH3 binding pocket of CED-9. This suppression suggests that CED-9 directly regulates DRP-1, a model supported by our finding that CED-9 activates the GTPase activity of human DRP1. Thus, CED-9 is capable of regulating the mitochondrial fission-fusion cycle but is not essential for either fission or fusion.

Links

PubMed PMC2785848 Online version:10.1242/jcs.032904

Keywords

Animals; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; GTP Phosphohydrolases/genetics; GTP Phosphohydrolases/metabolism; Homeostasis/physiology; Humans; Membrane Fusion/physiology; Microtubule-Associated Proteins/genetics; Microtubule-Associated Proteins/metabolism; Mitochondria/genetics; Mitochondria/metabolism; Mitochondrial Membranes/metabolism; Mitochondrial Proteins/genetics; Mitochondrial Proteins/metabolism; Models, Biological; Proto-Oncogene Proteins c-bcl-2/genetics; Proto-Oncogene Proteins c-bcl-2/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:CED9

enables

GO:0005096: GTPase activator activity

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_input:(UniProtKB:O00429)

Seeded From UniProt

complete

CAEEL:Q8WQC9

involved_in

GO:0000266: mitochondrial fission

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • occurs_in:(WBbt:0006804)
  • happens_during:(WBls:0000041)

Seeded From UniProt

complete

CAEEL:Q8WQC9

involved_in

GO:0000266: mitochondrial fission

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00000423

P

Seeded From UniProt

complete

CAEEL:CED9

enables

GO:0005096: GTPase activator activity

ECO:0000314: direct assay evidence used in manual assertion

F

has_direct_input:(UniProtKB:O00429)

Seeded From UniProt

complete

CAEEL:CED9

involved_in

GO:0010636: positive regulation of mitochondrial fusion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • occurs_in:(WBbt:0006804)
  • happens_during:(WBls:0000041)

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.