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PMID:18820465

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Citation

Page, SL, Nielsen, RJ, Teeter, K, Lake, CM, Ong, S, Wright, KR, Dean, KL, Agne, D, Gilliland, WD and Hawley, RS A germline clone screen for meiotic mutants in Drosophila melanogaster. Fly (Austin) 1:172-81

Abstract

Using an FLP/FRT-based method to create germline clones, we screened Drosophila chromosome arms 2L and 3R for new female meiotic mutants. The screen was designed to recover mutants with severe effects on meiotic exchange and/or segregation. This screen yielded 11 new mutants, including six alleles of previously known meiotic genes (c(2)M and ald/mps1). The remaining five mutants appear to define at least four new genes whose ablation results in severe meiotic defects. Three of the novel meiotic mutants were identified at the molecular level. Two of these, mcm5(A7) and trem(F9), define roles in meiotic recombination, while a third, cona(A12), is important for synaptonemal complex assembly. Surprisingly, five of the nine mutants for which the lesion has been identified at the molecular level are not the result of mutations characteristic of EMS mutagenesis, but rather due to the insertion of the transposable element Doc. This study demonstrates the utility of germline clone-based screens for the discovery of strong meiotic mutants, including mutations in essential genes, and the use of molecular genetic techniques to map the loci.

Links

PubMed

Keywords

Alleles; Animals; Crosses, Genetic; Drosophila melanogaster/genetics; Female; Genes, Insect; Genetic Testing/methods; Germ-Line Mutation; Male; Meiosis/genetics; Phenotype

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:A0A0B4KG66

involved_in

GO:0051321: meiotic cell cycle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8SXZ3

involved_in

GO:0051321: meiotic cell cycle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VDQ4

involved_in

GO:0051321: meiotic cell cycle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VE78

involved_in

GO:0007130: synaptonemal complex assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VEH1

involved_in

GO:0051321: meiotic cell cycle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:MCM5

involved_in

GO:0051321: meiotic cell cycle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VJL5

involved_in

GO:0051321: meiotic cell cycle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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