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PMID:18820069
| Citation |
Nicholson, WL (2008) The Bacillus subtilis ydjL (bdhA) gene encodes acetoin reductase/2,3-butanediol dehydrogenase. Appl. Environ. Microbiol. 74:6832-8 |
|---|---|
| Abstract |
Bacillus subtilis is capable of producing 2,3-butanediol from acetoin by fermentation, but to date, the gene encoding the enzyme responsible, acetoin reductase/2,3-butanediol dehydrogenase (AR/BDH), has remained unknown. A search of the B. subtilis genome database with the amino acid sequences of functional AR/BDHs from Saccharomyces cerevisiae and Bacillus cereus resulted in the identification of a highly similar protein encoded by the B. subtilis ydjL gene. A knockout strain carrying a ydjL::cat insertion mutation was constructed, which (i) abolished 2,3-butanediol production in early stationary phase, (ii) produced no detectable AR or BDH activity in vitro, and (iii) accumulated the precursor acetoin in early stationary phase. The ydjL::cat mutation also affected the kinetics of lactate but not acetate production during stationary-phase cultivation with glucose under oxygen limitation. A very small amount of 2,3-butanediol was detected in very-late-stationary-phase (96-hour) cultures of the ydjL::cat mutant, suggesting the existence of a second gene encoding a minor AR activity. From the data, it is proposed that the major AR/BDH-encoding gene ydjL be renamed bdhA. |
| Links |
PubMed PMC2583490 Online version:10.1128/AEM.00881-08 |
| Keywords |
Acetic Acid/metabolism; Acetoin/metabolism; Alcohol Oxidoreductases/genetics; Alcohol Oxidoreductases/metabolism; Bacillus cereus/genetics; Bacillus subtilis/enzymology; Bacillus subtilis/genetics; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Butylene Glycols/metabolism; Gene Deletion; Glucose/metabolism; Lactic Acid/metabolism; Mutagenesis, Insertional; Oxidoreductases/genetics; Oxidoreductases/metabolism; Oxygen/metabolism; Saccharomyces cerevisiae/genetics |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0000721: (R,R)-butanediol dehydrogenase activity |
ECO:0000315: |
F |
Table 3 shows that when the gene ydjL is mutated no BDH activity is detected. |
complete | ||||
|
enables |
GO:0000721: (R,R)-butanediol dehydrogenase activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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