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PMID:18799757

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Citation

Cao, W, Aghajanian, HK, Haig-Ladewig, LA and Gerton, GL (2009) Sorbitol can fuel mouse sperm motility and protein tyrosine phosphorylation via sorbitol dehydrogenase. Biol. Reprod. 80:124-33

Abstract

Energy sources that can be metabolized to yield ATP are essential for normal sperm functions such as motility. Two major monosaccharides, sorbitol and fructose, are present in semen. Furthermore, sorbitol dehydrogenase (SORD) can convert sorbitol to fructose, which can then be metabolized via the glycolytic pathway in sperm to make ATP. Here we characterize Sord mRNA and SORD expression during mouse spermatogenesis and examine the ability of sorbitol to support epididymal sperm motility and tyrosine phosphorylation. Sord mRNA levels increased during the course of spermatogenic differentiation. SORD protein, however, was first detected at the condensing spermatid stage. By indirect immunofluorescence, SORD was present along the length of the flagella of caudal epididymal sperm. Furthermore, immunoelectron microscopy showed that SORD was associated with mitochondria and the plasma membranes of sperm. Sperm incubated with sorbitol maintained motility, indicating that sorbitol was utilized as an energy source. Sorbitol, as well as glucose and fructose, were not essential to induce hyperactive motility. Protein tyrosine phosphorylation increased in a similar manner when sorbitol was substituted for glucose in the incubation medium used for sperm capacitation. These results indicate that sorbitol can serve as an alternative energy source for sperm motility and protein tyrosine phosphorylation.

Links

PubMed PMC2804811 Online version:10.1095/biolreprod.108.068882

Keywords

Animals; Blotting, Western; Fluorescent Antibody Technique, Indirect; L-Iditol 2-Dehydrogenase/biosynthesis; L-Iditol 2-Dehydrogenase/genetics; L-Iditol 2-Dehydrogenase/metabolism; Male; Mice; Microscopy, Immunoelectron; Phosphorylation; RNA, Messenger/biosynthesis; RNA, Messenger/genetics; Reverse Transcriptase Polymerase Chain Reaction; Sorbitol/metabolism; Sperm Motility/physiology; Sperm Tail/enzymology; Sperm Tail/physiology; Sperm Tail/ultrastructure; Spermatogenesis/physiology; Spermatozoa/enzymology; Spermatozoa/metabolism; Spermatozoa/ultrastructure; Tyrosine/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:DHSO

involved_in

GO:0030317: flagellated sperm motility

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:DHSO

located_in

GO:0005739: mitochondrion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:DHSO

located_in

GO:0031514: motile cilium

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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