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PMID:18784652

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Citation

Fischer, SE, Butler, MD, Pan, Q and Ruvkun, G (2008) Trans-splicing in C. elegans generates the negative RNAi regulator ERI-6/7. Nature 455:491-6

Abstract

Mutations that enhance the response to double-stranded RNA (dsRNA) have revealed components of the RNA interference (RNAi) pathway or related small RNA pathways. To explore these small RNA pathways, we screened for Caenorhabditis elegans mutants displaying an enhanced response to exogenous dsRNAs. Here we describe the isolation of mutations in two adjacent, divergently transcribed open reading frames (eri-6 and eri-7) that fail to complement. eri-6 and eri-7 produce separate pre-messenger RNAs (pre-mRNAs) that are trans-spliced to form a functional mRNA, eri-6/7. Trans-splicing of eri-6/7 is mediated by a direct repeat that flanks the eri-6 gene. Adenosine to inosine editing within untranslated regions of eri-6 and eri-7 pre-mRNAs reveals a double-stranded pre-mRNA intermediate, forming in the nucleus before splicing occurs. The ERI-6/7 protein is a superfamily I helicase that both negatively regulates the exogenous RNAi pathway and functions in an endogenous RNAi pathway.

Links

PubMed PMC2756026 Online version:10.1038/nature07274

Keywords

Animals; Caenorhabditis elegans/classification; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; DNA Helicases/genetics; DNA Helicases/metabolism; Genes, Helminth/genetics; Genetic Complementation Test; Molecular Sequence Data; Mutation/genetics; Open Reading Frames/genetics; RNA Interference/physiology; Repetitive Sequences, Nucleic Acid/genetics; Trans-Splicing

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:B5TRE1

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:B5TRE1

enables

GO:0004386: helicase activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P38935

F

Seeded From UniProt

complete

CAEEL:B5TRE1

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:B5TRE1

involved_in

GO:0016246: RNA interference

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:B5TRE1

enables

GO:0008026: ATP-dependent helicase activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P38935

F

Seeded From UniProt

complete

Notes

See also

References

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