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PMID:18775315

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Citation

Cai, L, Makhov, AM, Schafer, DA and Bear, JE (2008) Coronin 1B antagonizes cortactin and remodels Arp2/3-containing actin branches in lamellipodia. Cell 134:828-42

Abstract

The dendritic actin network generated by the Arp2/3 complex in lamellipodia underlies formation of protrusions, directional sensing, and migration. While the generation of this network is well studied, the mechanisms regulating network disassembly are poorly understood. We report that Coronin 1B disassembles Arp2/3-containing actin filament branches by inducing Arp2/3 dissociation. This activity is antagonized by Cortactin, a filament branch stabilizer. Consistent with this biochemical competition, depletion of both proteins partially rescues defects in lamellipodial dynamics observed upon depletion of either protein alone. Coronin 1B targets actin branches in a manner that is mutually exclusive with the Arp2/3 complex and alters the branch angle. We conclude that Coronin 1B replaces the Arp2/3 complex at actin filament branches as the dendritic network matures and drives the turnover of branched actin networks.

Links

PubMed PMC2570342 Online version:10.1016/j.cell.2008.06.054

Keywords

4-Butyrolactone/analogs & derivatives; 4-Butyrolactone/metabolism; Actin-Related Protein 2/metabolism; Actin-Related Protein 3/metabolism; Actins/metabolism; Animals; Cell Line; Cortactin/metabolism; Embryo, Mammalian/cytology; Fibroblasts; Humans; Mice; Pseudopodia; Rats

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:ARC1B

enables

GO:0044877: protein-containing complex binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:1305848
RGD:619839

F

Seeded From UniProt

complete

RAT:ARC1B

enables

GO:0044877: protein-containing complex binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:1305848
RGD:2382

F

Seeded From UniProt

complete

RAT:COR1B

enables

GO:0071933: Arp2/3 complex binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:1305848
RGD:2155

F

Seeded From UniProt

complete

RAT:ARPC2

enables

GO:0044877: protein-containing complex binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:2155
RGD:619839

F

Seeded From UniProt

complete

RAT:ARPC2

enables

GO:0044877: protein-containing complex binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:2155
RGD:2382

F

Seeded From UniProt

complete

RAT:SRC8

enables

GO:0071933: Arp2/3 complex binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:1305848
RGD:2155

F

Seeded From UniProt

complete

HUMAN:COR1B

located_in

GO:0005884: actin filament

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:COR1B

involved_in

GO:0030036: actin cytoskeleton organization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:COR1B

GO:0030036: actin cytoskeleton organization

ECO:0000314:

P

According to Figure 2C, in the presence of 50 nM Coronin 1B, total actin filament was reduced and branching was less extensive than in the control reactions.

complete
CACAO 7800

HUMAN:COR1B

involved_in

GO:0090135: actin filament branching

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:COR1B

enables

GO:0071933: Arp2/3 complex binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:COR1B

involved_in

GO:0034316: negative regulation of Arp2/3 complex-mediated actin nucleation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:COR1B

part_of

GO:0005884: actin filament

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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