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PMID:18768136

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Citation

Galganska, H, Budzinska, M, Wojtkowska, M and Kmita, H (2008) Redox regulation of protein expression in Saccharomyces cerevisiae mitochondria: possible role of VDAC. Arch. Biochem. Biophys. 479:39-45

Abstract

Using Saccharomyces cerevisiae mutants depleted of either isoform of VDAC (voltage dependent anion selective channel) we studied the role of the cytosol and mitochondria redox states in regulation of the expression levels of some mitochondrial proteins. The studied proteins are MnSOD and subunits of the protein import machinery of the mitochondrial outer membrane, i.e. Tom70, Tom40 and Tob55 (Sam50). We have shown that both the cytosol and mitochondria redox states depend on the presence of a given VDAC isoform. The cytosol redox state is mediated by VDAC1, although VDAC2 has a quantitative effect, whereas the mitochondria redox state depends on the presence of both VDAC isoforms. Moreover, we have shown that the cytosol redox status but not the mitochondrial one is decisive for the expression levels of the studied mitochondrial proteins. Thus, expression levels of some mitochondrial proteins is influenced by VDAC and this regulatory process at least partially does not require its channel activity as VDAC2 does not form a channel. Thus, VDAC can be regarded as a participant of signaling pathways in S. cerevisiae cells.

Links

PubMed Online version:10.1016/j.abb.2008.08.010

Keywords

Membrane Proteins/metabolism; Membrane Transport Proteins/metabolism; Mitochondria/genetics; Mitochondria/metabolism; Mitochondrial Membrane Transport Proteins/metabolism; Mitochondrial Proteins/metabolism; Oxidation-Reduction; Protein Isoforms; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Superoxide Dismutase/metabolism; Voltage-Dependent Anion Channel 1/genetics; Voltage-Dependent Anion Channel 1/metabolism; Voltage-Dependent Anion Channel 2/genetics; Voltage-Dependent Anion Channel 2/metabolism; Voltage-Dependent Anion Channels/genetics; Voltage-Dependent Anion Channels/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:VDAC1

involved_in

GO:0045454: cell redox homeostasis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:VDAC2

involved_in

GO:0045454: cell redox homeostasis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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