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Hontz, RD, French, SL, Oakes, ML, Tongaonkar, P, Nomura, M, Beyer, AL and Smith, JS (2008) Transcription of multiple yeast ribosomal DNA genes requires targeting of UAF to the promoter by Uaf30. Mol. Cell. Biol. 28:6709-19


Upstream activating factor (UAF) is a multisubunit complex that functions in the activation of ribosomal DNA (rDNA) transcription by RNA polymerase I (Pol I). Cells lacking the Uaf30 subunit of UAF reduce the rRNA synthesis rate by approximately 70% compared to wild-type cells and produce rRNA using both Pol I and Pol II. Miller chromatin spreads demonstrated that even though there is an overall reduction in rRNA synthesis in uaf30 mutants, the active rDNA genes in such strains are overloaded with polymerases. This phenotype was specific to defects in Uaf30, as mutations in other UAF subunits resulted in a complete absence of rDNA genes with high or even modest Pol densities. The lack of Uaf30 prevented UAF from efficiently binding to the rDNA promoter in vivo, leading to an inability to activate a large number of rDNA genes. The relatively few genes that did become activated were highly transcribed, apparently to compensate for the reduced rRNA synthesis capacity. The results show that Uaf30p is a key targeting factor for the UAF complex that facilitates activation of a large proportion of rDNA genes in the tandem array.


PubMed PMC2573240 Online version:10.1128/MCB.00703-08


DNA, Ribosomal/genetics; Genes, Fungal; Mutation/genetics; Promoter Regions, Genetic/genetics; Protein Binding; Protein Subunits/metabolism; RNA Polymerase I/metabolism; RNA Polymerase II/metabolism; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins/metabolism; Transcription Factors/metabolism; Transcription, Genetic



Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status



GO:0001181: RNA polymerase I general transcription initiation factor activity

ECO:0000315: mutant phenotype evidence used in manual assertion


Seeded From UniProt




GO:0042790: nucleolar large rRNA transcription by RNA polymerase I

ECO:0000315: mutant phenotype evidence used in manual assertion


Seeded From UniProt


See also


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