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PMID:18765566

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Citation

Sato, M, Grant, BD, Harada, A and Sato, K (2008) Rab11 is required for synchronous secretion of chondroitin proteoglycans after fertilization in Caenorhabditis elegans. J. Cell. Sci. 121:3177-86

Abstract

We previously identified a novel type of caveolin-enriched secretory vesicle in Caenorhabditis elegans oocytes. These vesicles undergo synchronous fusion with the plasma membrane immediately after fertilization, suggesting that they could be cortical granules that have been described in diverse animal species. Here, we report that these vesicles are indeed cortical granules, delivering essential chondroitin proteoglycans and mucin-like glycoproteins to the early embryonic extracellular matrices (ECMs). Furthermore, we have found that the small GTPase RAB-11 and the target-SNARE SYN-4 are required for cortical granule excoytosis after fertilization. In oocytes, SYN-4 localizes mainly to the plasma membrane, whereas GFP::RAB-11 accumulates transiently on the cortical granules during ovulation, immediately prior to fertilization. Importantly, cytokinesis defects in early embryos are commonly observed after depletion of either rab-11 or syn-4, producing a phenotype very similar to that observed after blockade of chondroitin synthesis. Taken together, our results indicate that at least part of the essential role for RAB-11 and SYN-4 in early embryogenesis is in the targeting of cortical granules to the plasma membrane during the precisely regulated secretion of ECM components.

Links

PubMed Online version:10.1242/jcs.034678

Keywords

Animals; Caenorhabditis elegans/cytology; Caenorhabditis elegans/embryology; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/metabolism; Caveolin 1/metabolism; Chondroitin/secretion; Cytokinesis; Cytoplasmic Granules/metabolism; Egg Shell/cytology; Egg Shell/metabolism; Embryo, Nonmammalian/cytology; Embryo, Nonmammalian/metabolism; Exocytosis; Extracellular Space/metabolism; Female; Fertilization/physiology; Green Fluorescent Proteins/metabolism; Oocytes/cytology; Protein Transport; Proteoglycans/secretion; R-SNARE Proteins/metabolism; Recombinant Fusion Proteins/metabolism; Sequence Homology, Amino Acid; Syndecan-4/metabolism; rab GTP-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:CDC16

involved_in

GO:0030703: eggshell formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:H2L2G6

located_in

GO:0060473: cortical granule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:H2L2G6

involved_in

GO:0046662: regulation of oviposition

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00000302

P

Seeded From UniProt

complete

CAEEL:RB11A

involved_in

GO:0000281: mitotic cytokinesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:RB11A

located_in

GO:0060473: cortical granule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:RB11A

involved_in

GO:0030703: eggshell formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:RB11A

involved_in

GO:0060471: cortical granule exocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:SYB1

located_in

GO:0060473: cortical granule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:STX4

located_in

GO:0060473: cortical granule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:CAV2

involved_in

GO:0046662: regulation of oviposition

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00000301

P

Seeded From UniProt

complete

CAEEL:CAV1

located_in

GO:0060473: cortical granule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:CAV1

involved_in

GO:0046662: regulation of oviposition

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00000302

P

Seeded From UniProt

complete

CAEEL:STX4

involved_in

GO:0032467: positive regulation of cytokinesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:STX4

part_of

GO:0060473: cortical granule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:STX4

involved_in

GO:0060471: cortical granule exocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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