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PMID:18758452

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Citation

Satoh, D, Sato, D, Tsuyama, T, Saito, M, Ohkura, H, Rolls, MM, Ishikawa, F and Uemura, T (2008) Spatial control of branching within dendritic arbors by dynein-dependent transport of Rab5-endosomes. Nat. Cell Biol. 10:1164-71

Abstract

Dendrites allow neurons to integrate sensory or synaptic inputs, and the spatial disposition and local density of branches within the dendritic arbor limit the number and type of inputs. Drosophila melanogaster dendritic arborization (da) neurons provide a model system to study the genetic programs underlying such geometry in vivo. Here we report that mutations of motor-protein genes, including a dynein subunit gene (dlic) and kinesin heavy chain (khc), caused not only downsizing of the overall arbor, but also a marked shift of branching activity to the proximal area within the arbor. This phenotype was suppressed when dominant-negative Rab5 was expressed in the mutant neurons, which deposited early endosomes in the cell body. We also showed that 1) in dendritic branches of the wild-type neurons, Rab5-containing early endosomes were dynamically transported and 2) when Rab5 function alone was abrogated, terminal branches were almost totally deleted. These results reveal an important link between microtubule motors and endosomes in dendrite morphogenesis.

Links

PubMed Online version:10.1038/ncb1776

Keywords

Animals; Axons/metabolism; Biological Transport; Carrier Proteins/metabolism; Clone Cells; Dendrites/metabolism; Drosophila Proteins/metabolism; Drosophila melanogaster/cytology; Drosophila melanogaster/genetics; Drosophila melanogaster/metabolism; Dyneins/metabolism; Endosomes/metabolism; Genes, Insect; Kinesin/metabolism; Morphogenesis; Mutation/genetics; Phenotype; rab5 GTP-Binding Proteins/deficiency; rab5 GTP-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:KINH

involved_in

GO:0007409: axonogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:KINH

involved_in

GO:0048813: dendrite morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:DYHC

part_of

GO:0030286: dynein complex

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0030276

C

Seeded From UniProt

complete

DROME:Q7KMS3

involved_in

GO:0048813: dendrite morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:LIS1

involved_in

GO:0048813: dendrite morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8IR93

involved_in

GO:0007409: axonogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8IR93

part_of

GO:0030286: dynein complex

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0261797

C

Seeded From UniProt

complete

DROME:Q8IR93

involved_in

GO:0048813: dendrite morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VZ20

involved_in

GO:0007409: axonogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VZ20

part_of

GO:0030286: dynein complex

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0261797

C

Seeded From UniProt

complete

DROME:Q9VZ20

involved_in

GO:0048813: dendrite morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9V3I2

involved_in

GO:0007409: axonogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9V3I2

involved_in

GO:0048813: dendrite morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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