GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:18723886

From GONUTS
Jump to: navigation, search
Citation

Yan, J, Huen, D, Morely, T, Johnson, G, Gubb, D, Roote, J and Adler, PN (2008) The multiple-wing-hairs gene encodes a novel GBD-FH3 domain-containing protein that functions both prior to and after wing hair initiation. Genetics 180:219-28

Abstract

The frizzled signaling/signal transduction pathway controls planar cell polarity (PCP) in both vertebrates and invertebrates. Epistasis experiments argue that in the Drosophila epidermis multiple wing hairs (mwh) acts as a downstream component of the pathway. The PCP proteins accumulate asymmetrically in pupal wing cells where they are thought to form distinct protein complexes. One is located on the distal side of wing cells and a second on the proximal side. This asymmetric protein accumulation is thought to lead to the activation of the cytoskeleton on the distal side, which in turn leads to each cell forming a single distally pointing hair. We identified mwh as CG13913, which encodes a novel G protein binding domain-formin homology 3 (GBD-FH3) domain protein. The Mwh protein accumulated on the proximal side of wing cells prior to hair formation. Unlike planar polarity proteins such as Frizzled or Inturned, Mwh also accumulated in growing hairs. This suggested that mwh had two temporally separate functions in wing development. Evidence for these two functions also came from temperature-shift experiments with a temperature-sensitive allele. Overexpression of Mwh inhibited hair initiation, thus Mwh acts as a negative regulator of the cytoskeleton. Our data argued early proximal Mwh accumulation restricts hair initiation to the distal side of wing cells and the later hair accumulation of Mwh prevents the formation of ectopic secondary hairs. This later function appears to be a feedback mechanism that limits cytoskeleton activation to ensure a single hair is formed.

Links

PubMed PMC2535676 Online version:10.1534/genetics.108.091314

Keywords

Actins/genetics; Alleles; Animals; Cloning, Molecular; Cytoskeleton/metabolism; Drosophila Proteins/genetics; Drosophila Proteins/physiology; Drosophila melanogaster/genetics; Drosophila melanogaster/physiology; Frizzled Receptors/genetics; GTP-Binding Proteins/metabolism; Models, Genetic; Mutation; Plasmids/metabolism; Protein Structure, Tertiary; Receptors, G-Protein-Coupled/genetics; Signal Transduction; Temperature; Wing/metabolism; Wing/pathology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:M9PDL4

involved_in

GO:0035320: imaginal disc-derived wing hair site selection

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:M9PDL4

involved_in

GO:0035317: imaginal disc-derived wing hair organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:M9PE53

involved_in

GO:0035320: imaginal disc-derived wing hair site selection

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:M9PE53

involved_in

GO:0035317: imaginal disc-derived wing hair organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9W0J2

involved_in

GO:0035320: imaginal disc-derived wing hair site selection

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9W0J2

involved_in

GO:0035317: imaginal disc-derived wing hair organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.