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PMID:18713317

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Citation

Jonas, K, Edwards, AN, Simm, R, Romeo, T, Römling, U and Melefors, O (2008) The RNA binding protein CsrA controls cyclic di-GMP metabolism by directly regulating the expression of GGDEF proteins. Mol. Microbiol. 70:236-57

Abstract

The carbon storage regulator CsrA is an RNA binding protein that controls carbon metabolism, biofilm formation and motility in various eubacteria. Nevertheless, in Escherichia coli only five target mRNAs have been shown to be directly regulated by CsrA at the post-transcriptional level. Here we identified two new direct targets for CsrA, ycdT and ydeH, both of which encode proteins with GGDEF domains. A csrA mutation caused mRNA levels of ycdT and ydeH to increase more than 10-fold. RNA mobility shift assays confirmed the direct and specific binding of CsrA to the mRNA leaders of ydeH and ycdT. Overexpression of ycdT and ydeH resulted in a more than 20-fold increase in the cellular concentration of the second messenger cyclic di-GMP (c-di-GMP), implying that both proteins possess diguanylate cyclase activity. Phenotypic characterization revealed that both proteins are involved in the regulation of motility in a c-di-GMP-dependent manner. CsrA was also found to regulate the expression of five additional GGDEF/EAL proteins and a csrA mutation led to modestly increased cellular levels of c-di-GMP. All together, these data demonstrate a global role for CsrA in the regulation of c-di-GMP metabolism by regulating the expression of GGDEF proteins at the post-transcriptional level.

Links

PubMed PMC2735045 Online version:10.1111/j.1365-2958.2008.06411.x

Keywords

Cyclic GMP/analogs & derivatives; Cyclic GMP/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genome, Bacterial; Genotype; Microscopy, Atomic Force; Mutagenesis, Site-Directed; Mutation; Oligonucleotide Array Sequence Analysis; Phenotype; Plasmids; Protein Binding; RNA Processing, Post-Transcriptional; RNA, Bacterial/metabolism; RNA, Messenger/metabolism; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Repressor Proteins/genetics; Repressor Proteins/metabolism; Reverse Transcriptase Polymerase Chain Reaction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:DGCZ

acts_upstream_of_or_within

GO:0051271: negative regulation of cellular component movement

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:DGCZ

enables

GO:0052621: diguanylate cyclase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:DGCZ

acts_upstream_of_or_within

GO:1902209: negative regulation of bacterial-type flagellum assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:DGCT

acts_upstream_of_or_within

GO:0051271: negative regulation of cellular component movement

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:DGCT

enables

GO:0052621: diguanylate cyclase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:YCDT

involved_in

GO:0051271: negative regulation of cellular component movement

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:YCDT

enables

GO:0052621: diguanylate cyclase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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