GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:18710660

From GONUTS
Jump to: navigation, search
Citation

Nichane, M, Ren, X, Souopgui, J and Bellefroid, EJ (2008) Hairy2 functions through both DNA-binding and non DNA-binding mechanisms at the neural plate border in Xenopus. Dev. Biol. 322:368-80

Abstract

The Xenopus helix-loop-helix transcription factor Hairy2 is essential for neural crest progenitor survival and maintains cells in a mitotic undifferentiated pre-neural crest state. However, its mode of action remains largely unknown. Here we show that a Hairy2 DNA-binding mutant is unable to promote cell survival and to upregulate the expression of early neural border genes but is capable to increase cell proliferation and to expand NC in late embryos. We found that Hairy2 transiently activates in a DNA-binding independent manner the expression of the Notch ligand Delta1 and that Delta1 is required for Hairy2 to promote cell proliferation and to expand NC. Finally, we provide evidence that Hairy2 induces Delta1 through the transcription factor Stat3. Together, these results suggest that Hairy2 has a dual mode of action and may function at the neural plate border through both a DNA-binding and a non-DNA-binding Stat3-Delta1 mediated mechanism.

Links

PubMed Online version:10.1016/j.ydbio.2008.07.026

Keywords

Animals; Basic Helix-Loop-Helix Transcription Factors/physiology; Cell Proliferation; DNA-Binding Proteins/physiology; Embryo, Nonmammalian/metabolism; Inhibitor of Differentiation Proteins/metabolism; Intracellular Signaling Peptides and Proteins; Membrane Proteins/metabolism; Neural Plate/embryology; Neural Plate/metabolism; Protein Structure, Tertiary; STAT3 Transcription Factor/metabolism; Up-Regulation; Xenopus/embryology; Xenopus/metabolism; Xenopus Proteins/metabolism; Xenopus Proteins/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

XENLA:HES4B

involved_in

GO:0014029: neural crest formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

XENLA:HES4B

involved_in

GO:0043066: negative regulation of apoptotic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

XENLA:HES4A

involved_in

GO:0014029: neural crest formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

XENLA:HES4A

involved_in

GO:0043066: negative regulation of apoptotic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

XENLA:ID3A

involved_in

GO:0014029: neural crest formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

XENLA:STA31

involved_in

GO:0014033: neural crest cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.