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PMID:18692037

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Citation

Owens, P, Bazzi, H, Engelking, E, Han, G, Christiano, AM and Wang, XJ (2008) Smad4-dependent desmoglein-4 expression contributes to hair follicle integrity. Dev. Biol. 322:156-66

Abstract

We have previously shown that keratinocyte-specific deletion of Smad4, a TGFbeta/Activin/BMP signaling mediator, results in a progressive alopecia. To further assess the molecular mechanisms of Smad4 loss-mediated alopecia, we examined expression levels of key molecules associated with hair follicle differentiation in Smad4-deleted skin. Among them, Desmoglein 4 (Dsg4) was down-regulated in Smad4-deleted skin prior to the onset of hair follicle abnormalities with gradual depletion coinciding with hair follicle degeneration. Chromatin immunoprecipitation (ChIP) assay showed that Smad4, together with the BMP mediators Smad1 and Smad5, but not the TGFbeta/Activin mediators Smad2 or Smad3, bound to the Smad Binding Element (SBE) of the Dsg4 promoter. A Dsg4 reporter assay revealed that Smad4 was required for the maximal transactivation of Dsg4 in cooperation with Smad1 and Smad5. Mutating the SBE of the Dsg4 promoter abrogated Smad4 transactivation of Dsg4. Furthermore, BMP ligands, but not ligands of TGFbeta and Activin, induced endogenous Dsg4 expression. Our data demonstrate that in the presence of Smad4, BMP signaling participated in transcriptional regulation of Dsg4. Thus, Smad4 loss-associated Dsg4 depletion contributed, at least in part, to hair follicles degeneration in Smad4 deficient skin.

Links

PubMed PMC2642977 Online version:10.1016/j.ydbio.2008.07.020

Keywords

Alopecia/genetics; Alopecia/metabolism; Alopecia/pathology; Animals; Bone Morphogenetic Proteins/metabolism; Cell Differentiation/genetics; Cells, Cultured; Desmogleins/genetics; Desmogleins/metabolism; Down-Regulation/genetics; Hair Follicle/metabolism; Hair Follicle/pathology; Keratinocytes/metabolism; Keratinocytes/pathology; Ligands; Mice; Mice, Knockout; Mice, Transgenic; Promoter Regions, Genetic/genetics; Regulatory Sequences, Nucleic Acid/genetics; Signal Transduction/genetics; Skin/pathology; Smad1 Protein/metabolism; Smad4 Protein/deficiency; Smad4 Protein/genetics; Smad4 Protein/metabolism; Smad5 Protein/metabolism; Transcriptional Activation/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:CADH1

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:17525)

Seeded From UniProt

complete

MOUSE:PCNA

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:SMAD1

enables

GO:0000978: RNA polymerase II cis-regulatory region sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

  • occurs_in:(EMAPA:17525)
  • has_input:(MGI:MGI:2661061)

Seeded From UniProt

complete

MOUSE:SMAD5

enables

GO:0000978: RNA polymerase II cis-regulatory region sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

  • occurs_in:(EMAPA:17525)
  • has_input:(MGI:MGI:2661061)

Seeded From UniProt

complete

MOUSE:SMAD4

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:SMAD4

acts_upstream_of_or_within

GO:0051797: regulation of hair follicle development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2386393

P

  • regulates_o_results_in_development_of:(EMAPA:17525)

Seeded From UniProt

complete

MOUSE:SMAD4

acts_upstream_of_or_within

GO:0048733: sebaceous gland development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2386393

P

  • results_in_development_of:(EMAPA:35754)

Seeded From UniProt

complete

MOUSE:SMAD4

acts_upstream_of_or_within

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2386393

P

  • regulates_o_occurs_in:(EMAPA:17525)
  • has_input:(MGI:MGI:2661061)

Seeded From UniProt

complete

MOUSE:K1C14

located_in

GO:0071944: cell periphery

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:DSG4

acts_upstream_of_or_within

GO:0001942: hair follicle development

ECO:0000314: direct assay evidence used in manual assertion

P

  • results_in_development_of:(EMAPA:17525)

Seeded From UniProt

complete

MOUSE:DSG4

acts_upstream_of_or_within

GO:0030509: BMP signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CADH1

part_of

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(EMAPA:17525)

Seeded From UniProt

complete

MOUSE:SMAD1

enables

GO:0000978: RNA polymerase II proximal promoter sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

  • occurs_in:(EMAPA:17525)
  • has_participant:(MGI:MGI:2661061)

Seeded From UniProt

complete

MOUSE:SMAD4

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:SMAD4

acts_upstream_of_or_within

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2386393

P

  • regulates_o_occurs_in:(EMAPA:17525)
  • has_regulation_target:(MGI:MGI:2661061)

Seeded From UniProt

complete

MOUSE:SMAD4

acts_upstream_of_or_within

GO:0048733: sebaceous gland development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2386393

P

results_in_development_of:(EMAPA:35754)

Seeded From UniProt

complete

MOUSE:SMAD4

acts_upstream_of_or_within

GO:0051797: regulation of hair follicle development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2386393

P

regulates_o_results_in_development_of:(EMAPA:17525)

Seeded From UniProt

complete

MOUSE:SMAD5

enables

GO:0000978: RNA polymerase II proximal promoter sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_participant:(MGI:MGI:2661061)
  • occurs_in:(EMAPA:17525)

Seeded From UniProt

complete

See also

References

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