PMID:18682278

Citation	Alonso-Espinaco, V, Elezgarai, I, Díez-García, J, Puente, N, Knöpfel, T and Grandes, P (2008) Subcellular localization of the voltage-gated potassium channels Kv3.1b and Kv3.3 in the cerebellar dentate nucleus of glutamic acid decarboxylase 67-green fluorescent protein transgenic mice. Neuroscience 155:1059-69
Abstract	Deep cerebellar dentate nuclei are in a key position to control motor planning as a result of an integration of cerebropontine inputs and hemispheric Purkinje neurons signals, and their influence through synaptic outputs onto extracerebellar hubs. GABAergic dentate neurons exhibit broader action potentials and slower afterhyperpolarization than non-GABAergic (presumably glutamatergic) neurons. Specific potassium channels may be involved in these distinct firing profiles, particularly, Kv3.1 and Kv3.3 subunits which rapidly activate at relatively positive potentials to support the generation of fast action potentials. To investigate the subcellular localization of Kv3.1b and Kv3.3 in GAD- and GAD+ dentate neurons of glutamic acid decarboxylase 67-green fluorescent protein (GAD67-GFP) knock-in mice a preembedding immunocytochemical method for electron microscopy was used. Kv3.1b and Kv3.3 were in membranes of cell somata, dendrites, axons and synaptic terminals of both GAD- and GAD+ dentate neurons. The vast majority of GAD- somatodendritic membrane segments domains labeled for Kv3.1b and Kv3.3 (96.1% and 84.7%, respectively) whereas 56.2% and 69.8% of GAD- axonal membrane segments were immunopositive for these subunits. Furthermore, density of Kv3.1b immunoparticles was much higher in GAD- somatodendritic than axonal domains. As to GAD+ neurons, only 70.6% and 50% of somatodendritic membrane segments, and 53.3% and 59.5% of axonal membranes exhibited Kv3.1b and Kv3.3 labeling, respectively. In contrast to GAD- cells, GAD+ cells exhibited a higher density labeling for both Kv3 subunits at their axonal than at their somatodendritic membranes. Taken together, Kv3.1b and Kv3.3 potassium subunits are expressed in both GAD- and GAD+ cells, albeit at different densities and distribution. They likely contribute to the distinct biophysical properties of both GAD- and GAD+ neurons in the dentate nucleus.
Links	PubMed Online version:10.1016/j.neuroscience.2008.07.014
Keywords	Animals; Cerebellar Nuclei/metabolism; Cerebellar Nuclei/ultrastructure; Gene Expression Regulation/genetics; Glutamate Decarboxylase/deficiency; Glutamate Decarboxylase/genetics; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Mice; Mice, Inbred C57BL; Mice, Transgenic; Microscopy, Immunoelectron/methods; Shaw Potassium Channels/metabolism; Shaw Potassium Channels/ultrastructure; Subcellular Fractions/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:KCNC1	located_in	GO:0030673: axolemma	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:KCNC1	located_in	GO:0032809: neuronal cell body membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:KCNC1	located_in	GO:0032590: dendrite membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:KCNC3	located_in	GO:0030673: axolemma	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:KCNC3	located_in	GO:0032809: neuronal cell body membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:KCNC3	located_in	GO:0032590: dendrite membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete

References

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