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PMID:18667600

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Citation

Paunescu, TG, Jones, AC, Tyszkowski, R and Brown, D (2008) V-ATPase expression in the mouse olfactory epithelium. Am. J. Physiol., Cell Physiol. 295:C923-30

Abstract

The vacuolar proton-pumping ATPase (V-ATPase) is responsible for the acidification of intracellular organelles and for the pH regulation of extracellular compartments. Because of the potential role of the latter process in olfaction, we examined the expression of V-ATPase in mouse olfactory epithelial (OE) cells. We report that V-ATPase is present in this epithelium, where we detected subunits ATP6V1A (the 70-kDa "A" subunit) and ATP6V1E1 (the ubiquitous 31-kDa "E" subunit isoform) in epithelial cells, nerve fiber cells, and Bowman's glands by immunocytochemistry. We also located both isoforms of the 56-kDa B subunit, ATP6V1B1 ("B1," typically expressed in epithelia specialized in regulated transepithelial proton transport) and ATP6V1B2 ("B2") in the OE. B1 localizes to the microvilli of the apical plasma membrane of sustentacular cells and to the lateral membrane in a subset of olfactory sensory cells, which also express carbonic anhydrase type IV, whereas B2 expression is stronger in the subapical domain of sustentacular cells. V-ATPase expression in mouse OE was further confirmed by immunoblotting. These findings suggest that V-ATPase may be involved in proton secretion in the OE and, as such, may be important for the pH homeostasis of the neuroepithelial mucous layer and/or for signal transduction in CO(2) detection.

Links

PubMed PMC2575830 Online version:10.1152/ajpcell.00237.2008

Keywords

Animals; Carbon Dioxide; Gene Deletion; Gene Expression Regulation/physiology; Mice; Olfactory Mucosa/metabolism; Protein Isoforms; Protein Subunits; Signal Transduction/physiology; Vacuolar Proton-Translocating ATPases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:VATA

located_in

GO:0005902: microvillus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:VATA

located_in

GO:0016324: apical plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:VATE1

located_in

GO:0005902: microvillus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:VATE1

located_in

GO:0016324: apical plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:VATB2

located_in

GO:0005902: microvillus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:VATB1

located_in

GO:0016328: lateral plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000207)

Seeded From UniProt

complete

MOUSE:VATB1

located_in

GO:0005902: microvillus

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0002167)

Seeded From UniProt

complete

MOUSE:VATB1

located_in

GO:0016324: apical plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0002167)

Seeded From UniProt

complete


See also

References

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