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PMID:18656261

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Citation

Holdorf, MM, Bennett, B, Crowder, MW and Makaroff, CA (2008) Spectroscopic studies on Arabidopsis ETHE1, a glyoxalase II-like protein. J. Inorg. Biochem. 102:1825-30

Abstract

ETHE1 (ethylmalonic encephalopathy protein 1) is a beta-lactamase fold-containing protein that is essential for the survival of a range of organisms. In spite of the apparent importance of this enzyme, very little is known about its function or biochemical properties. In this study Arabidopsis ETHE1 was over-expressed and purified and shown to bind tightly to 1.2+/-0.2 equivalents of iron. (1)H NMR and EPR studies demonstrate that the predominant oxidation state of Fe in ETHE1 is Fe(II), and NMR studies confirm that two histidines are bound to Fe(II). EPR studies show that there is no antiferromagnetically coupled Fe(III)Fe(II) center in ETHE1. Gel filtration studies reveal that ETHE1 is a dimer in solution, which is consistent with previous crystallographic studies. Although very similar in terms of amino acid sequence to glyoxalase II, ETHE1 exhibits no thioester hydrolase activity, and activity screening assays reveal that ETHE1 exhibits low level esterase activity. Taken together, ETHE1 is a novel, mononuclear Fe(II)-containing member of the beta-lactamase fold superfamily.

Links

PubMed PMC2556177 Online version:10.1016/j.jinorgbio.2008.06.003

Keywords

Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Dimerization; Electron Spin Resonance Spectroscopy/methods; Ferrous Compounds/chemistry; Iron/metabolism; Magnetic Resonance Spectroscopy/methods; Manganese/metabolism; Oxidation-Reduction; Protein Binding; Thiolester Hydrolases/chemistry; Thiolester Hydrolases/genetics; Thiolester Hydrolases/metabolism; Zinc/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:ETHE1

enables

GO:0016788: hydrolase activity, acting on ester bonds

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:ETHE1

NOT|enables

GO:0047951: glutathione thiolesterase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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