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PMID:18618131

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Citation

Georgas, K, Rumballe, B, Wilkinson, L, Chiu, HS, Lesieur, E, Gilbert, T and Little, MH (2008) Use of dual section mRNA in situ hybridisation/immunohistochemistry to clarify gene expression patterns during the early stages of nephron development in the embryo and in the mature nephron of the adult mouse kidney. Histochem. Cell Biol. 130:927-42

Abstract

The kidney is the most complex organ within the urogenital system. The adult mouse kidney contains in excess of 8,000 mature nephrons, each of which can be subdivided into a renal corpuscle and 14 distinct tubular segments. The histological complexity of this organ can make the clarification of the site of gene expression by in situ hybridisation difficult. We have defined a panel of seven antibodies capable of identifying the six stages of early nephron development, the tubular nephron segments and the components of the renal corpuscle within the embryonic and adult mouse kidney. We have analysed in detail the protein expression of Wt1, Calb1 Aqp1, Aqp2 and Umod using these antibodies. We have then coupled immunohistochemistry with RNA in situ hybridisation in order to precisely identify the expression pattern of different genes, including Wnt4, Umod and Spp1. This technique will be invaluable for examining at high resolution, the structure of both the developing and mature nephron where standard in situ hybridisation and histological techniques are insufficient. The use of this technique will enhance the expression analyses of genes which may be involved in nephron formation and the function of the mature nephron in the mouse.

Links

PubMed Online version:10.1007/s00418-008-0454-3

Keywords

Animals; Aquaporin 1/analysis; Aquaporin 2/analysis; Calcium-Binding Protein, Vitamin D-Dependent; Calcium-Binding Proteins/analysis; Female; Gene Expression Regulation, Developmental; Immunohistochemistry; In Situ Hybridization; Male; Mice; Mucoproteins/analysis; Mucoproteins/genetics; Nephrons/chemistry; Nephrons/embryology; Nephrons/growth & development; Nerve Tissue Proteins/analysis; Osteopontin/genetics; RNA, Messenger/analysis; Uromodulin; WT1 Proteins/analysis; Wnt Proteins/genetics; Wnt4 Protein

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:CALB1

involved_in

GO:0072286: metanephric connecting tubule development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CALB1

involved_in

GO:0072221: metanephric distal convoluted tubule development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CALB1

involved_in

GO:0072205: metanephric collecting duct development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CALB1

involved_in

GO:0035502: metanephric part of ureteric bud development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:AQP2

involved_in

GO:0072205: metanephric collecting duct development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:UROM

involved_in

GO:0072233: metanephric thick ascending limb development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:UROM

involved_in

GO:0072221: metanephric distal convoluted tubule development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:UROM

involved_in

GO:0072218: metanephric ascending thin limb development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:WT1

involved_in

GO:0072015: glomerular visceral epithelial cell development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:WT1

involved_in

GO:0072278: metanephric comma-shaped body morphogenesis

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:WT1

involved_in

GO:0072284: metanephric S-shaped body morphogenesis

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:AQP1

involved_in

GO:0072220: metanephric descending thin limb development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:AQP1

involved_in

GO:0072232: metanephric proximal convoluted tubule segment 2 development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:AQP1

involved_in

GO:0072230: metanephric proximal straight tubule development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:AQP1

involved_in

GO:0072239: metanephric glomerulus vasculature development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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