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PMID:18614048

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Citation

Saguez, C, Schmid, M, Olesen, JR, Ghazy, MA, Qu, X, Poulsen, MB, Nasser, T, Moore, C and Jensen, TH (2008) Nuclear mRNA surveillance in THO/sub2 mutants is triggered by inefficient polyadenylation. Mol. Cell 31:91-103

Abstract

The yeast THO complex and the associated RNA helicase Sub2p are important mRNP maturation factors. Transcripts produced in THO/sub2 mutants are subject to degradation by a surveillance mechanism that involves the nuclear RNA exosome. Here we show that inefficient polyadenylation forms the basis of this accelerated mRNA decay. A genetic screen reveals extensive interactions between deletions of THO subunits and mRNA 3' end processing mutants. Nuclear run-ons strengthen this link by showing premature transcription termination close to polyadenylation sites in THO/sub2 mutants in vivo. Moreover, in vitro, pre-mRNA substrates are poorly polyadenylated and consequently unstable in extracts from THO/sub2 mutant strains. Decreased polyadenylation correlates with a specific downregulation of the poly(A)-polymerase cofactor Fip1p by the ubiquitin/proteasome pathway. Both polyadenylation defects and Fip1p instability depend on the nuclear exosome component Rrp6p and its activator Trf4p. We suggest that removal of aberrant mRNA is facilitated by direct regulation of polyadenylation activity.

Links

PubMed Online version:10.1016/j.molcel.2008.04.030

Keywords

Adenosine Triphosphatases/metabolism; Amino Acid Transport Systems/metabolism; Cell Nucleus/metabolism; Codon, Nonsense; Down-Regulation; Multiprotein Complexes/metabolism; Mutation/genetics; Poly A/metabolism; Polyadenylation; Proteasome Endopeptidase Complex/metabolism; RNA Precursors/metabolism; RNA Stability; RNA, Fungal/metabolism; RNA, Messenger; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae Proteins/metabolism; Transcription, Genetic; Ubiquitin/metabolism; mRNA Cleavage and Polyadenylation Factors/metabolism

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

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