GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:18599512

From GONUTS
Jump to: navigation, search
Citation

Ouellet, J, Li, S and Roy, R (2008) Notch signalling is required for both dauer maintenance and recovery in C. elegans. Development 135:2583-92

Abstract

The Notch signalling pathway is conserved among higher metazoans and is used repeatedly throughout development to specify distinct cell fates among populations of equipotent cells. Mounting evidence suggests that Notch signalling may also be crucial in neuronal function in postmitotic, differentiated neurons. Here, we demonstrate a novel role for the canonical Notch signalling pathway in postmitotic neurons during a specialised ;diapause-like' post-embryonic developmental stage in C. elegans called dauer. Our data suggest that cell signalling downstream of the developmental decision to enter dauer leads to the activation of Notch-responding genes in postmitotic neurons. Consistent with this, we demonstrate that glp-1, one of the two C. elegans Notch receptors, and its ligand lag-2 are expressed in neurons during the dauer stage, and both genes are required to maintain this stage in a daf-7/TGFbeta dauer constitutive background. Our genetic data also suggest that a second Notch receptor, lin-12, functions upstream of, or in parallel with, insulin-like signalling components in response to replete growth conditions to promote dauer recovery. Based on our findings, cues associated with the onset of dauer ultimately trigger a glp-1-dependent Notch signalling cascade in neurons to maintain this developmental state. Then, as growth conditions improve, activation of the LIN-12 Notch receptor cooperates with the insulin-like signalling pathway to signal recovery from the dauer stage.

Links

PubMed Online version:10.1242/dev.012435

Keywords

Animals; Binding Sites; Caenorhabditis elegans/cytology; Caenorhabditis elegans/genetics; Caenorhabditis elegans/growth & development; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Forkhead Transcription Factors/metabolism; Gene Expression Regulation, Developmental; Insulin/metabolism; Ligands; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mitosis; Mutation/genetics; Neurons/cytology; Neurons/metabolism; Receptors, Glucagon/genetics; Receptors, Glucagon/metabolism; Receptors, Notch/genetics; Receptors, Notch/metabolism; Signal Transduction; Transcription Factors/genetics; Transcription Factors/metabolism; Transforming Growth Factor beta/genetics; Transforming Growth Factor beta/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:GLP1

involved_in

GO:0043055: maintenance of dauer

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00000903

P

Seeded From UniProt

complete

CAEEL:LIN12

involved_in

GO:0043054: dauer exit

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00000903

P

Seeded From UniProt

complete

CAEEL:LAG2

involved_in

GO:0043055: maintenance of dauer

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00000903

P

Seeded From UniProt

complete

CAEEL:UN130

involved_in

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00095029

P

Seeded From UniProt

complete

CAEEL:UN130

enables

GO:0003714: transcription corepressor activity

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00095029

F

Seeded From UniProt

complete

CAEEL:LAG1

involved_in

GO:0043054: dauer exit

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00000903

P

Seeded From UniProt

complete

CAEEL:G5ECC6

involved_in

GO:0043055: maintenance of dauer

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00000903

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.