PMID:18593560

Citation	Bondue, A, Lapouge, G, Paulissen, C, Semeraro, C, Iacovino, M, Kyba, M and Blanpain, C (2008) Mesp1 acts as a master regulator of multipotent cardiovascular progenitor specification. Cell Stem Cell 3:69-84
Abstract	During embryonic development, multipotent cardiovascular progenitor cells are specified from early mesoderm. Using mouse ESCs in which gene expression can be temporally regulated, we have found that transient expression of Mesp1 dramatically accelerates and enhances multipotent cardiovascular progenitor specification through an intrinsic and cell autonomous mechanism. Genome-wide transcriptional analysis indicates that Mesp1 rapidly activates and represses a discrete set of genes, and chromatin immunoprecipitation shows that Mesp1 directly binds to regulatory DNA sequences located in the promoter of many key genes in the core cardiac transcriptional machinery, resulting in their rapid upregulation. Mesp1 also directly represses the expression of key genes regulating other early mesoderm and endoderm cell fates. Our results demonstrate that Mesp1 acts as a key regulatory switch during cardiovascular specification, residing at the top of the hierarchy of the gene network responsible for cardiovascular cell-fate determination.
Links	PubMed Online version:10.1016/j.stem.2008.06.009
Keywords	Animals; Basic Helix-Loop-Helix Transcription Factors/genetics; Basic Helix-Loop-Helix Transcription Factors/physiology; Cardiovascular System/cytology; Cell Differentiation/physiology; Embryonic Development; Gene Expression Regulation, Developmental; Heart/embryology; Heart/physiology; Helix-Loop-Helix Motifs/physiology; Homeostasis; Mice; Myocardium/cytology; Pluripotent Stem Cells/cytology; Pluripotent Stem Cells/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:TBXT	involved_in	GO:0090009: primitive streak formation	ECO:0000303: author statement without traceable support used in manual assertion		P		Seeded From UniProt	complete
MOUSE:MESP1	enables	GO:0000987: cis-regulatory region sequence-specific DNA binding	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
MOUSE:MESP1	involved_in	GO:0042664: negative regulation of endodermal cell fate specification	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:MESP1	involved_in	GO:0045747: positive regulation of Notch signaling pathway	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:MESP1	involved_in	GO:0045892: negative regulation of transcription, DNA-templated	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:MESP1	involved_in	GO:0045893: positive regulation of transcription, DNA-templated	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:MESP1	involved_in	GO:0051155: positive regulation of striated muscle cell differentiation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:MESP1	located_in	GO:0005634: nucleus	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:MESP1	involved_in	GO:0060913: cardiac cell fate determination	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:MESP1	involved_in	GO:0070368: positive regulation of hepatocyte differentiation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:MESP1	involved_in	GO:0035481: positive regulation of Notch signaling pathway involved in heart induction	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:MESP1	involved_in	GO:0042662: negative regulation of mesodermal cell fate specification	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:MESP1	involved_in	GO:0003139: secondary heart field specification	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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