GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:18590717

From GONUTS
Jump to: navigation, search
Citation

Sperber, SM and Dawid, IB (2008) barx1 is necessary for ectomesenchyme proliferation and osteochondroprogenitor condensation in the zebrafish pharyngeal arches. Dev. Biol. 321:101-10

Abstract

Barx1 modulates cellular adhesion molecule expression and participates in specification of tooth-types, but little is understood of its role in patterning the pharyngeal arches. We examined barx1 expression during zebrafish craniofacial development and performed a functional analysis using antisense morpholino oligonucleotides. Barx1 is expressed in the rhombencephalic neural crest, the pharyngeal arches, the pectoral fin buds and the gut in contrast to its paralogue barx2, which is most prominently expressed in the arch epithelium. Additionally, barx1 transient expression was observed in the posterior lateral line ganglia and developing trunk/tail. We show that Barx1 is necessary for proliferation of the arch osteochondrogenic progenitors, and that morphants exhibit diminished and dysmorphic arch cartilage elements due to reductions in chondrocyte differentiation and condensation. Attenuation of Barx1 results in lost arch expression of osteochondrogenic markers col2a1, runx2a and chondromodulin, as well as odontogenic marker dlx2b. Further, loss of barx1 positively influenced gdf5 and chordin, markers of jaw joint patterning. FGF signaling is required for maintaining barx1 expression, and that ectopic BMP4 induces expression of barx1 in the intermediate region of the second pharyngeal arch. Together, these results indicate an essential role for barx1 at early stages of chondrogenesis within the developing zebrafish viscerocranium.

Links

PubMed PMC2669285 Online version:10.1016/j.ydbio.2008.06.004

Keywords

Animals; Animals, Genetically Modified; Bone Morphogenetic Protein 4; Bone Morphogenetic Proteins/metabolism; Branchial Region/embryology; Cell Proliferation; Chondrogenesis; Embryo, Nonmammalian/metabolism; Fibroblast Growth Factors/metabolism; Homeodomain Proteins/genetics; Humans; Mice; Neural Crest/embryology; Transcription Factors/genetics; Transcription Factors/metabolism; Zebrafish/embryology; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:BARX1

involved_in

GO:0002053: positive regulation of mesenchymal cell proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-081120-1,ZFIN:ZDB-MRPHLNO-081120-2

P

Seeded From UniProt

complete

DANRE:BARX1

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-081120-2

P

Seeded From UniProt

complete

DANRE:BARX1

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-081120-1,ZFIN:ZDB-MRPHLNO-081120-2

P

Seeded From UniProt

complete

DANRE:BARX1

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-081120-1

P

Seeded From UniProt

complete

DANRE:BARX1

involved_in

GO:0060037: pharyngeal system development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-081120-2

P

Seeded From UniProt

complete

DANRE:BARX1

involved_in

GO:0060037: pharyngeal system development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-081120-1,ZFIN:ZDB-MRPHLNO-081120-2

P

Seeded From UniProt

complete

DANRE:BARX1

involved_in

GO:0060037: pharyngeal system development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-081120-1

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.