PMID:18590697

Citation	Euro, L, Belevich, G, Verkhovsky, MI, Wikström, M and Verkhovskaya, M (2008) Conserved lysine residues of the membrane subunit NuoM are involved in energy conversion by the proton-pumping NADH:ubiquinone oxidoreductase (Complex I). Biochim. Biophys. Acta 1777:1166-72
Abstract	Analysis of the amino acid sequences of subunits NuoM and NuoN in the membrane domain of Complex I revealed a clear common pattern, including two lysines that are predicted to be located within the membrane, and which are important for quinone reductase activity. Site-directed mutations of the amino acid residues E144, K234, K265 and W243 in this pattern were introduced into the chromosomal gene nuoM of Escherichia coli Complex I. The activity of mutated Complex I was studied in both membranes and in purified Complex I. The quinone reductase activity was practically lost in K234A, K234R and E144A, decreased in W243A and K265A but unchanged in E144D. Complex I from all these mutants contained 1 mol tightly bound ubiquinone per mol FMN like wild type enzyme. The mutant enzymes E144D, W243A and K265A had wild type sensitivity to rolliniastatin and complete proton-pumping efficiency of Complex I. Remarkably, the subunits NuoL and NuoH in the membrane domain also appear to contain conserved lysine residues in transmembrane helices, which may give a clue of the mechanism of proton translocation. A tentative principle of proton translocation by Complex I is suggested based on electrostatic interactions of lysines in the membrane subunits.
Links	PubMed Online version:10.1016/j.bbabio.2008.06.001
Keywords	Amino Acid Sequence; Cell Membrane/metabolism; Conserved Sequence; Electron Transport Complex I/chemistry; Electron Transport Complex I/metabolism; Energy Transfer; Escherichia coli/enzymology; Escherichia coli/growth & development; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Lysine/metabolism; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutant Proteins/metabolism; Mutation/genetics; NADH Dehydrogenase/chemistry; NADH Dehydrogenase/metabolism; Phenotype; Protein Structure, Secondary; Protein Subunits/chemistry; Protein Subunits/metabolism; Sequence Analysis, Protein
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ECOLI:NUOM	acts_upstream_of_or_within	GO:0015990: electron transport coupled proton transport	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ECOLI:NUOM	involved_in	GO:0015990: electron transport coupled proton transport	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.