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PMID:18548008

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Citation

Yamauchi, T, Ishidao, T, Nomura, T, Shinagawa, T, Tanaka, Y, Yonemura, S and Ishii, S (2008) A B-Myb complex containing clathrin and filamin is required for mitotic spindle function. EMBO J. 27:1852-62

Abstract

B-Myb is one member of the vertebrate Myb family of transcription factors and is ubiquitously expressed. B-Myb activates transcription of a group of genes required for the G2/M cell cycle transition by forming the dREAM/Myb-MuvB-like complex, which was originally identified in Drosophila. Mutants of zebrafish B-myb and Drosophila myb exhibit defects in cell cycle progression and genome instability. Although the genome instability caused by a loss of B-Myb has been speculated to be due to abnormal cell cycle progression, the precise mechanism remains unknown. Here, we have purified a B-Myb complex containing clathrin and filamin (Myb-Clafi complex). This complex is required for normal localization of clathrin at the mitotic spindle, which was previously reported to stabilize kinetochore fibres. The Myb-Clafi complex is not tightly associated with the mitotic spindles, suggesting that this complex ferries clathrin to the mitotic spindles. Thus, identification of the Myb-Clafi complex reveals a previously unrecognized function of B-Myb that may contribute to its role in chromosome stability, possibly, tumour suppression.

Links

PubMed PMC2486420 Online version:10.1038/emboj.2008.118

Keywords

Animals; Clathrin/isolation & purification; Clathrin/metabolism; Contractile Proteins/isolation & purification; Contractile Proteins/metabolism; Fibroblasts/metabolism; Genomic Instability; HeLa Cells; Humans; Mice; Microfilament Proteins/isolation & purification; Microfilament Proteins/metabolism; Mitosis; Mitotic Spindle Apparatus/metabolism; Multiprotein Complexes/isolation & purification; Multiprotein Complexes/metabolism; Proto-Oncogene Proteins c-myb/isolation & purification; Proto-Oncogene Proteins c-myb/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:MYB

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q00610

F

Seeded From UniProt

complete

MOUSE:MYBB

acts_upstream_of_or_within

GO:0090307: mitotic spindle assembly

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:MYBB

part_of

GO:0031523: Myb complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CLH1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P10242

F

Seeded From UniProt

complete

HUMAN:Q49AL0

involved_in

GO:0090307: mitotic spindle assembly

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:Q49AL0

part_of

GO:0031523: Myb complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:CLH1

acts_upstream_of_or_within

GO:0090307: mitotic spindle assembly

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:Q49AL0

P

Seeded From UniProt

complete

MOUSE:CLH1

part_of

GO:0031523: Myb complex

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:Q49AL0

C

Seeded From UniProt

complete

HUMAN:FLNA

involved_in

GO:0090307: mitotic spindle assembly

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:FLNA

part_of

GO:0031523: Myb complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:FLNA

acts_upstream_of_or_within

GO:0090307: mitotic spindle assembly

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:P21333

P

Seeded From UniProt

complete

MOUSE:FLNA

part_of

GO:0031523: Myb complex

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:P21333

C

Seeded From UniProt

complete


See also

References

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