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PMID:18515076

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Citation

Sayegh, J and Clarke, SG (2008) Hsl7 is a substrate-specific type II protein arginine methyltransferase in yeast. Biochem. Biophys. Res. Commun. 372:811-5

Abstract

The Saccharomyces cerevisiae protein Hsl7 is a regulator of the Swe1 protein kinase in cell cycle checkpoint control. Hsl7 has been previously described as a type III protein arginine methyltransferase, catalyzing the formation of omega-monomethylarginine residues on non-physiological substrates. However, we show here that Hsl7 can also display type II activity, generating symmetric dimethylarginine residues on calf thymus histone H2A. Symmetric dimethylation is only observed when enzyme and the methyl-accepting substrate were incubated for extended times. We confirmed the Hsl7-dependent formation of symmetric dimethylarginine by amino acid analysis and thin layer chromatography with wild-type and mutant recombinant enzymes expressed from both bacteria and yeast. This result is significant because no type II activity has been previously demonstrated in S. cerevisiae. We also show that Hsl7 has little or no activity on GST-GAR, a commonly used substrate for protein arginine methyltransferases, and only minimal activity on myelin basic protein. This enzyme thus may only recognize only a small subset of potential substrate proteins in yeast, in contrast to the situation with Rmt1, the major type I methyltransferase.

Links

PubMed PMC2505109 Online version:10.1016/j.bbrc.2008.05.121

Keywords

Animals; Arginine/analogs & derivatives; Arginine/analysis; Arginine/metabolism; Cattle; Histones/metabolism; Methylation; Protein Kinases/chemistry; Protein Kinases/genetics; Protein Kinases/metabolism; Protein-Arginine N-Methyltransferases/chemistry; Protein-Arginine N-Methyltransferases/genetics; Protein-Arginine N-Methyltransferases/metabolism; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Substrate Specificity

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:HMT1

involved_in

GO:0018216: peptidyl-arginine methylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:HSL7

enables

GO:0035241: protein-arginine omega-N monomethyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:HSL7

enables

GO:0035243: protein-arginine omega-N symmetric methyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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