PMID:1850412

Citation	Yu, XP, Mocharla, H, Hustmyer, FG and Manolagas, SC (1991) Vitamin D receptor expression in human lymphocytes. Signal requirements and characterization by western blots and DNA sequencing. J. Biol. Chem. 266:7588-95
Abstract	The signals controlling the expression of the receptor protein for 1 alpha,25-dihydroxyvitamin D3 in normal human lymphocytes and the relationship of this protein to the classical vitamin D receptor were examined. Lymphocytes activated with the OKT3 antibody to the T-cell antigen receptor expressed fewer binding sites as compared to lymphocytes that were activated by the polyclonal activator phytohemagglutinin (PHA). However, combination of OKT3 and phorbol myristate acetate produced a concentration of binding sites similar to the PHA-activated cells. The receptor from OKT3 and OKT3 + phorbol myristate acetate-activated lymphocytes exhibited decreased binding to DNA-cellulose compared to PHA-activated lymphocytes. In lymphocytes activated either by PHA or OKT3 (but not in resting cells), a 50-kDa species cross-reacting with a monoclonal antibody against the intestinal vitamin D receptor was detected. Finally, RNA from activated lymphocytes was amplified by polymerase chain reaction using oligonucleotide primers flanking the 196 base pair long region encoding the DNA-binding domain of the human intestinal receptor. The amplified product showed an identical nucleotide sequence to the DNA-binding domain of the human intestinal receptor. These findings suggest that expression of the 1,25-(OH)2D3 receptor in lymphocytes is triggered by distinct and contingent signals, and that the protein and the mRNA encoding it are identical to the classical vitamin D receptor.
Links	PubMed
Keywords	Antibodies, Monoclonal/diagnostic use; Base Sequence; Blotting, Western; Calcitriol/metabolism; Chromatography, Liquid; DNA/genetics; Female; Flow Cytometry; Humans; Lymphocyte Activation; Lymphocytes/drug effects; Lymphocytes/metabolism; Male; Molecular Sequence Data; Phytohemagglutinins/pharmacology; Polymerase Chain Reaction; Receptors, Calcitriol; Receptors, Steroid/genetics; Receptors, Steroid/metabolism; Tetradecanoylphorbol Acetate/pharmacology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:VDR		GO:0046649: lymphocyte activation	ECO:0000270:		P		FIG. 7. Western blot analysis of 1,25-(OH)2Ds receptor in activated human lymphocytes.	complete CACAO 3509

References

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