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PMID:18502617

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Citation

Sugiyama, Y, Nishimura, A and Ohno, S (2008) Symmetrically dividing cell specific division axes alteration observed in proteasome depleted C. elegans embryo. Mech. Dev. 125:743-55

Abstract

A fertilised Caenorhabditis elegans embryo shows an invariable pattern of cell division and forms a multicellular body where each cell locates to a defined position. Mitotic spindle orientation is determined by several preceding events including the migration of duplicated centrosomes on a nucleus and the rotation of nuclear-centrosome complex. Cell polarity is the dominant force driving nuclear-centrosome rotation and setting the mitotic spindle axis in parallel with the polarity axis during asymmetric cell division. It is reasonable that there is no nuclear-centrosome rotation in symmetrically dividing blastomeres, but the mechanism(s) which suppress rotation in these cells have been proposed because the rotations occur in some polarity defect embryos. Here we show the nuclear-centrosome rotation can be induced by depletion of RPN-2, a regulatory subunit of the proteasome. In these embryos, cell polarity is established normally and both asymmetrically and symmetrically dividing cells are generated through asymmetric cell divisions. The nuclear-centrosome rotations occurred normally in the asymmetrically dividing cell lineage, but also induced in symmetrically dividing daughter cells. Interestingly, we identified RPN-2 as a binding protein of PKC-3, one of critical elements for establishing cell polarity during early asymmetric cell divisions. In addition to asymmetrically dividing cells, PKC-3 is also expressed in symmetrically dividing cells and a role to suppress nuclear-centrosome rotation has been anticipated. Our data suggest that the expression of RPN-2 is involved in the mechanism to suppress nuclear-centrosome rotation in symmetrically dividing cells and it may work in cooperation with PKC-3.

Links

PubMed Online version:10.1016/j.mod.2008.04.002

Keywords

Animals; Body Patterning/physiology; Caenorhabditis elegans/embryology; Caenorhabditis elegans/physiology; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/physiology; Cell Division/physiology; Cell Polarity/physiology; Centrosome/physiology; Embryo, Nonmammalian/physiology; Proteasome Endopeptidase Complex/genetics; Proteasome Endopeptidase Complex/physiology; Protein Subunits/genetics; Protein Subunits/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:PSMD1

located_in

GO:0031965: nuclear membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:PSMD1

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:PSMD1

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:PSMD1

enables

GO:0005080: protein kinase C binding

ECO:0000353: physical interaction evidence used in manual assertion

WB:WBGene00004034

F

Seeded From UniProt

complete

CAEEL:PSMD1

located_in

GO:0072686: mitotic spindle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:PSMD1

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:PSMD1

located_in

GO:0005938: cell cortex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:KPC3

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

WB:WBGene00004459

F

Seeded From UniProt

complete

CAEEL:KPC3

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

WB:WBGene00003921

F

Seeded From UniProt

complete

CAEEL:PAR6

enables

GO:0005080: protein kinase C binding

ECO:0000353: physical interaction evidence used in manual assertion

WB:WBGene00004034

F

Seeded From UniProt

complete


See also

References

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