GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:18502617
| Citation |
Sugiyama, Y, Nishimura, A and Ohno, S (2008) Symmetrically dividing cell specific division axes alteration observed in proteasome depleted C. elegans embryo. Mech. Dev. 125:743-55 |
|---|---|
| Abstract |
A fertilised Caenorhabditis elegans embryo shows an invariable pattern of cell division and forms a multicellular body where each cell locates to a defined position. Mitotic spindle orientation is determined by several preceding events including the migration of duplicated centrosomes on a nucleus and the rotation of nuclear-centrosome complex. Cell polarity is the dominant force driving nuclear-centrosome rotation and setting the mitotic spindle axis in parallel with the polarity axis during asymmetric cell division. It is reasonable that there is no nuclear-centrosome rotation in symmetrically dividing blastomeres, but the mechanism(s) which suppress rotation in these cells have been proposed because the rotations occur in some polarity defect embryos. Here we show the nuclear-centrosome rotation can be induced by depletion of RPN-2, a regulatory subunit of the proteasome. In these embryos, cell polarity is established normally and both asymmetrically and symmetrically dividing cells are generated through asymmetric cell divisions. The nuclear-centrosome rotations occurred normally in the asymmetrically dividing cell lineage, but also induced in symmetrically dividing daughter cells. Interestingly, we identified RPN-2 as a binding protein of PKC-3, one of critical elements for establishing cell polarity during early asymmetric cell divisions. In addition to asymmetrically dividing cells, PKC-3 is also expressed in symmetrically dividing cells and a role to suppress nuclear-centrosome rotation has been anticipated. Our data suggest that the expression of RPN-2 is involved in the mechanism to suppress nuclear-centrosome rotation in symmetrically dividing cells and it may work in cooperation with PKC-3. |
| Links |
PubMed Online version:10.1016/j.mod.2008.04.002 |
| Keywords |
Animals; Body Patterning/physiology; Caenorhabditis elegans/embryology; Caenorhabditis elegans/physiology; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/physiology; Cell Division/physiology; Cell Polarity/physiology; Centrosome/physiology; Embryo, Nonmammalian/physiology; Proteasome Endopeptidase Complex/genetics; Proteasome Endopeptidase Complex/physiology; Protein Subunits/genetics; Protein Subunits/physiology |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
CAEEL:PSMD1 |
located_in |
GO:0031965: nuclear membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
CAEEL:PSMD1 |
located_in |
GO:0005634: nucleus |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
CAEEL:PSMD1 |
located_in |
GO:0005737: cytoplasm |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
CAEEL:PSMD1 |
enables |
GO:0005080: protein kinase C binding |
ECO:0000353: physical interaction evidence used in manual assertion |
WB:WBGene00004034 |
F |
Seeded From UniProt |
complete | |
|
CAEEL:PSMD1 |
located_in |
GO:0072686: mitotic spindle |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
CAEEL:PSMD1 |
located_in |
GO:0005886: plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
CAEEL:PSMD1 |
located_in |
GO:0005938: cell cortex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
CAEEL:KPC3 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
WB:WBGene00004459 |
F |
Seeded From UniProt |
complete | |
|
CAEEL:KPC3 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
WB:WBGene00003921 |
F |
Seeded From UniProt |
complete | |
|
CAEEL:PAR6 |
enables |
GO:0005080: protein kinase C binding |
ECO:0000353: physical interaction evidence used in manual assertion |
WB:WBGene00004034 |
F |
Seeded From UniProt |
complete | |
See also
References
See Help:References for how to manage references in GONUTS.
