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PMID:18497747

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Citation

Kanamori, T, Inoue, T, Sakamoto, T, Gengyo-Ando, K, Tsujimoto, M, Mitani, S, Sawa, H, Aoki, J and Arai, H (2008) Beta-catenin asymmetry is regulated by PLA1 and retrograde traffic in C. elegans stem cell divisions. EMBO J. 27:1647-57

Abstract

Asymmetric division is an important property of stem cells. In Caenorhabditis elegans, the Wnt/beta-catenin asymmetry pathway determines the polarity of most asymmetric divisions. The Wnt signalling components such as beta-catenin localize asymmetrically to the cortex of mother cells to produce two distinct daughter cells. However, the molecular mechanism to polarize them remains to be elucidated. Here, we demonstrate that intracellular phospholipase A(1) (PLA(1)), a poorly characterized lipid-metabolizing enzyme, controls the subcellular localizations of beta-catenin in the terminal asymmetric divisions of epithelial stem cells (seam cells). In mutants of ipla-1, a single C. elegans PLA(1) gene, cortical beta-catenin is delocalized and the asymmetry of cell-fate specification is disrupted in the asymmetric divisions. ipla-1 mutant phenotypes are rescued by expression of ipla-1 in seam cells in a catalytic activity-dependent manner. Furthermore, our genetic screen utilizing ipla-1 mutants reveals that reduction of endosome-to-Golgi retrograde transport in seam cells restores normal subcellular localization of beta-catenin to ipla-1 mutants. We propose that membrane trafficking regulated by ipla-1 provides a mechanism to control the cortical asymmetry of beta-catenin.

Links

PubMed PMC2396877 Online version:10.1038/emboj.2008.102

Keywords

Animals; Biological Transport; Caenorhabditis elegans/cytology; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/metabolism; Cell Count; Cell Division; Cell Lineage; Cell Polarity; Cytoskeletal Proteins/metabolism; Female; Genes, Helminth; Genes, Suppressor; Guanine Nucleotide Exchange Factors; Mitotic Spindle Apparatus/metabolism; Mutant Proteins/isolation & purification; Mutant Proteins/metabolism; Mutation/genetics; Phenotype; Phospholipases A1/metabolism; Stem Cells/cytology; Subcellular Fractions/metabolism; Vulva/cytology; beta Catenin/metabolism; rab GTP-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:PLA1

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:PLA1

involved_in

GO:0032880: regulation of protein localization

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00275268

P

Seeded From UniProt

complete

CAEEL:PLA1

located_in

GO:0005829: cytosol

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:PLA1

involved_in

GO:0031161: phosphatidylinositol catabolic process

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q8NEL9

P

Seeded From UniProt

complete

CAEEL:PLA1

involved_in

GO:0009786: regulation of asymmetric cell division

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00275268

P

Seeded From UniProt

complete

CAEEL:PLA1

enables

GO:0008970: phospholipase A1 activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q8NEL9

F

Seeded From UniProt

complete

CAEEL:O45434

located_in

GO:0005794: Golgi apparatus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:Q7JL36

located_in

GO:0005794: Golgi apparatus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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