GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:18485748

From GONUTS
Jump to: navigation, search
Citation

Luan, Y, Kong, L, Howell, DR, Ilalov, K, Fajardo, M, Bai, XH, Di Cesare, PE, Goldring, MB, Abramson, SB and Liu, CJ (2008) Inhibition of ADAMTS-7 and ADAMTS-12 degradation of cartilage oligomeric matrix protein by alpha-2-macroglobulin. Osteoarthr. Cartil. 16:1413-20

Abstract

As we previously reported, ADAMTS-7 and ADAMTS-12, two members of ADAMTS (a disintegrin and metalloprotease with thrombospondin motifs) family, degrade cartilage oligomeric matrix protein (COMP) in vitro and are significantly induced in the cartilage and synovium of arthritic patients [Liu CJ, Kong W, Ilalov K, Yu S, Xu K, Prazak L, et al. ADAMTS-7: a metalloproteinase that directly binds to and degrades cartilage oligomeric matrix protein. FASEB J 2006;20(7):988-90; Liu CJ, Kong W, Xu K, Luan Y, Ilalov K, Sehgal B, et al. ADAMTS-12 associates with and degrades cartilage oligomeric matrix protein. J Biol Chem 2006;281(23):15800-8]. The purpose of this study was to determine (1) whether cleavage activity of ADAMTS-7 and ADAMTS-12 of COMP are associated with COMP degradation in osteoarthritis (OA); (2) whether alpha-2-macroglobulin (a(2)M) is a novel substrate for ADAMTS-7 and ADAMTS-12; and (3) whether a(2)M inhibits ADAMTS-7 or ADAMTS-12 cleavage of COMP.

Links

PubMed PMC2574789 Online version:10.1016/j.joca.2008.03.017

Keywords

ADAM Proteins/antagonists & inhibitors; Adult; Blotting, Western; Cartilage Oligomeric Matrix Protein; Cartilage, Articular/metabolism; Extracellular Matrix Proteins/metabolism; Glycoproteins/metabolism; Humans; Matrilin Proteins; Middle Aged; Polymerase Chain Reaction; RNA, Messenger/metabolism; alpha-Macroglobulins/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:COMP

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P58397

F

Seeded From UniProt

complete

HUMAN:COMP

enables

GO:0002020: protease binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P58397

F

Seeded From UniProt

complete

HUMAN:COMP

enables

GO:0002020: protease binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9UKP4

F

Seeded From UniProt

complete

HUMAN:ATS12

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P49747

F

Seeded From UniProt

complete

HUMAN:ATS12

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P01023

F

Seeded From UniProt

complete

HUMAN:ATS12

enables

GO:0004222: metalloendopeptidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_input:(UniProtKB:P49747)|has_input:(UniProtKB:P01023)

Seeded From UniProt

complete

HUMAN:ATS12

involved_in

GO:0051603: proteolysis involved in cellular protein catabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • has_input:(UniProtKB:P49747)
  • part_of:(GO:0071347)
  • occurs_in:(UBERON:0000313)|has_input(UniProtKB:P49747)
  • part_of:(GO:0071356)
  • occurs_in:(UBERON:0000313)|has_input(UniProtKB:P01023)

Seeded From UniProt

complete

HUMAN:ATS12

involved_in

GO:0071356: cellular response to tumor necrosis factor

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:ATS12

involved_in

GO:0071347: cellular response to interleukin-1

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:ATS7

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P01023

F

Seeded From UniProt

complete

HUMAN:ATS7

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P49747

F

Seeded From UniProt

complete

HUMAN:ATS7

enables

GO:0004222: metalloendopeptidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_input:(UniProtKB:P49747)|has_input:(UniProtKB:P01023)

Seeded From UniProt

complete

HUMAN:ATS7

involved_in

GO:0051603: proteolysis involved in cellular protein catabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • has_input:(UniProtKB:P49747)
  • part_of:(GO:0071347)
  • occurs_in:(UBERON:0000313)|has_input(UniProtKB:P49747)
  • part_of:(GO:0071356)
  • occurs_in:(UBERON:0000313)|has_input(UniProtKB:P01023)

Seeded From UniProt

complete

HUMAN:ATS7

involved_in

GO:0071356: cellular response to tumor necrosis factor

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:ATS7

involved_in

GO:0071347: cellular response to interleukin-1

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:A2MG

enables

GO:0002020: protease binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P58397

F

Seeded From UniProt

complete

HUMAN:A2MG

enables

GO:0002020: protease binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9UKP4

F

Seeded From UniProt

complete

HUMAN:B4DKJ3

enables

GO:0002020: protease binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9UKP4

F

Seeded From UniProt

complete

HUMAN:B4DKJ3

enables

GO:0002020: protease binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P58397

F

Seeded From UniProt

complete

Notes

See also

References

See Help:References for how to manage references in GONUTS.