GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:18466303

From GONUTS
Jump to: navigation, search
Citation

Lee, J, Oh, M, Park, H and Lee, I (2008) SOC1 translocated to the nucleus by interaction with AGL24 directly regulates leafy. Plant J. 55:832-43

Abstract

SUMMARY: Suppressor of overexpression of constans1 (SOC1) is one of the flowering pathway integrators and regulates the expression of LEAFY (LFY), which links floral induction and floral development. However, the mechanism by which SOC1, a MADS box protein, regulates LFY has proved elusive. Here, we show that SOC1 directly binds to the distal and proximal region of the LFY promoter where critical cis-elements are located. Intragenic suppressor mutant analysis shows that a missense mutation in the MADS box of SOC1 causes loss of binding to the LFY promoter as well as suppression of the flowering promotion function. The full-length SOC1 protein locates in the cytoplasm if expressed alone in protoplast transient expression assay, but relocates to the nucleus if expressed with AGAMOUS-LIKE 24 (AGL24), another flowering pathway integrator and a MADS box protein. The domain analysis shows that co-localization of SOC1 and AGL24 is mediated by the MADS box and the intervening region of SOC1. Finally, we show that LFY is expressed only in those tissues where SOC1 and AGL24 expressions overlap. Thus, we propose that heterodimerization of SOC1 and AGL24 is a key mechanism in activating LFY expression.

Links

PubMed Online version:10.1111/j.1365-313X.2008.03552.x

Keywords

Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cell Nucleus/genetics; Cell Nucleus/metabolism; Chromatin Immunoprecipitation; DNA, Plant/genetics; Gene Expression Regulation, Plant; Genes, Plant; MADS Domain Proteins/genetics; MADS Domain Proteins/metabolism; Mutation, Missense; Phenotype; Plants, Genetically Modified/genetics; Plants, Genetically Modified/metabolism; Plasmids; Promoter Regions, Genetic; Protein Transport; Transcription Factors/genetics; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:SOC1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O82794

F

Seeded From UniProt

complete

ARATH:SOC1

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:SOC1

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:SOC1

involved_in

GO:0009739: response to gibberellin

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AGL24

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O64645

F

Seeded From UniProt

complete

ARATH:AGL24

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.