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PMID:18459044

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Citation

Kim, DW, Lee, HN, Song, JE, Jung, KJ, Yang, WM, Kwon, K, Jeon, GS, Lee, YH, Chung, CK and Cho, SS (2008) Expression of transferrin binding protein in the capillaries of the brain in the developing chick embryo. Neurochem. Res. 33:2288-93

Abstract

Transferrin-binding protein (TfBP) has been shown to be a novel protein, structurally related to the chicken heat shock protein 108. The physiological function of this protein, however, has not yet been established. Antiserum to TfBP selectively stains transferrin- and iron-rich oligodendrocytes and choroidal epithelium in the adult and embryonic chick brain, suggesting a role for this protein in transferrin and iron storage in these cells. In this study, we further demonstrate TfBP-immunoreactivity (IR) in the blood vessels of the embryonic chick central nervous system. A strong TfBP-IR was present in blood vessels from E6, declined from E10 and was absent by E18. Thus, the expression of the TfBP in the blood vessels precedes its expression in the oligodendrocytes. At the subcellular level, TfBP-IR was confined to the cytoplasm of capillary pericytes while the Tf-receptor IR was associated with the capillary endothelium of the brain. The up-regulated expression of TfBP, together with the Tf-receptor of the brain capillaries, suggests that pericytes may be associated with the high iron uptake required for the metabolic demands of the developing brain.

Links

PubMed Online version:10.1007/s11064-008-9716-7

Keywords

Animals; Brain/embryology; Brain/metabolism; Capillaries/metabolism; Chick Embryo; Immunohistochemistry; Microscopy, Fluorescence; Microscopy, Immunoelectron; Transferrin-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:ENPL

located_in

GO:0048471: perinuclear region of cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:ENPL

involved_in

GO:0006880: intracellular sequestering of iron ion

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:ENPL

part_of

GO:0048471: perinuclear region of cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:TRFE

involved_in

GO:0006880: intracellular sequestering of iron ion

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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