GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:18458156

From GONUTS
Jump to: navigation, search
Citation

Shang, Y, Yoshida, T, Amendt, BA, Martin, JF and Owens, GK (2008) Pitx2 is functionally important in the early stages of vascular smooth muscle cell differentiation. J. Cell Biol. 181:461-73

Abstract

Mechanisms that control vascular smooth muscle cell (SMC) differentiation are poorly understood. We identify Pitx2 as a previously unknown homeodomain transcription factor that is rapidly induced in an in vitro model of SMC differentiation from multipotent stem cells. Pitx2 induces expression of multiple SMC differentiation marker genes by binding to a TAATC(C/T) cis-element, by interacting with serum response factor, and by increasing histone acetylation levels within the promoters of SMC differentiation marker genes. Suppression of Pitx2 reduces expression of SMC differentiation marker genes in the early stages of SMC differentiation in vitro, whereas Prx1, another homeodomain protein, regulates SMC differentiation marker genes in fully differentiated SMCs. Pitx2, but not Prx1, knockout mouse embryos exhibit impaired induction of SMC differentiation markers in the dorsal aorta and branchial arch arteries. Our results demonstrate that Pitx2 functions to regulate the early stages of SMC differentiation.

Links

PubMed PMC2364692 Online version:10.1083/jcb.200711145

Keywords

Animals; Biological Markers/metabolism; Cell Differentiation/physiology; Cells, Cultured; Embryo, Mammalian/anatomy & histology; Embryo, Mammalian/physiology; Embryonic Stem Cells/cytology; Embryonic Stem Cells/physiology; Gene Expression Regulation; Histone Deacetylase 2; Histone Deacetylases/genetics; Histone Deacetylases/metabolism; Histones/metabolism; Homeodomain Proteins/genetics; Homeodomain Proteins/metabolism; Mice; Mice, Knockout; Muscle, Smooth, Vascular/cytology; Myocytes, Smooth Muscle/cytology; Myocytes, Smooth Muscle/physiology; Promoter Regions, Genetic; RNA, Small Interfering/genetics; RNA, Small Interfering/metabolism; Repressor Proteins/genetics; Repressor Proteins/metabolism; Serum Response Factor/genetics; Serum Response Factor/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription, Genetic; Two-Hybrid System Techniques; p300-CBP Transcription Factors/genetics; p300-CBP Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:HDAC3

enables

GO:0031490: chromatin DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:PITX2

acts_upstream_of_or_within

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:PITX2

acts_upstream_of_or_within

GO:0035886: vascular associated smooth muscle cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2136268

P

  • occurs_in:(EMAPA:16039)

Seeded From UniProt

complete

MOUSE:SRF

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P97474-3

F

Seeded From UniProt

complete

MOUSE:SRF

enables

GO:0031490: chromatin DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_input:(MGI:MGI:87909)

Seeded From UniProt

complete

MOUSE:HDAC2

enables

GO:0031490: chromatin DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:EP300

enables

GO:0031490: chromatin DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.