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PMID:18456666

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Citation

Wagner, S, Pop, OI, Pop, O, Haan, GJ, Baars, L, Koningstein, G, Klepsch, MM, Genevaux, P, Luirink, J and de Gier, JW (2008) Biogenesis of MalF and the MalFGK(2) maltose transport complex in Escherichia coli requires YidC. J. Biol. Chem. 283:17881-90

Abstract

The polytopic inner membrane protein MalF is a constituent of the MalFGK(2) maltose transport complex in Escherichia coli. We have studied the biogenesis of MalF using a combination of in vivo and in vitro approaches. MalF is targeted via the SRP pathway to the Sec/YidC insertion site. Despite close proximity of nascent MalF to YidC during insertion, YidC is not required for the insertion of MalF into the membrane. However, YidC is required for the stability of MalF and the formation of the MalFGK(2) maltose transport complex. Our data indicate that YidC supports the folding of MalF into a stable conformation before it is incorporated into the maltose transport complex.

Links

PubMed Online version:10.1074/jbc.M801481200

Keywords

ATP-Binding Cassette Transporters/metabolism; ATP-Binding Cassette Transporters/physiology; Biological Transport; Cell Membrane/metabolism; Escherichia coli/metabolism; Escherichia coli Proteins/metabolism; Escherichia coli Proteins/physiology; Gene Expression Regulation, Bacterial; Maltose/metabolism; Membrane Transport Proteins/metabolism; Membrane Transport Proteins/physiology; Models, Biological; Monosaccharide Transport Proteins/metabolism; Monosaccharide Transport Proteins/physiology; Plasmids/metabolism; Protein Binding; Protein Conformation; Protein Folding

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:MALF

located_in

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:YIDC

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:YIDC

located_in

GO:0016021: integral component of membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:YIDC

involved_in

GO:0006457: protein folding

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:YIDC

involved_in

GO:0065003: protein-containing complex assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:MALG

located_in

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:MALK

located_in

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:OXAA

part_of

GO:0016021: integral component of membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:OXAA

involved_in

GO:0065003: protein-containing complex assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:OXAA

involved_in

GO:0006457: protein folding

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:OXAA

part_of

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:MALK

part_of

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

See also

References

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