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PMID:18451108

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Citation

Merz, K, Hondele, M, Goetze, H, Gmelch, K, Stoeckl, U and Griesenbeck, J (2008) Actively transcribed rRNA genes in S. cerevisiae are organized in a specialized chromatin associated with the high-mobility group protein Hmo1 and are largely devoid of histone molecules. Genes Dev. 22:1190-204

Abstract

Synthesis of ribosomal RNAs (rRNAs) is the major transcriptional event in proliferating cells. In eukaryotes, ribosomal DNA (rDNA) is transcribed by RNA polymerase I from a multicopy locus coexisting in at least two different chromatin states. This heterogeneity of rDNA chromatin has been an obstacle to defining its molecular composition. We developed an approach to analyze differential protein association with each of the two rDNA chromatin states in vivo in the yeast Saccharomyces cerevisiae. We demonstrate that actively transcribed rRNA genes are largely devoid of histone molecules, but instead associate with the high-mobility group protein Hmo1.

Links

PubMed PMC2335315 Online version:10.1101/gad.466908

Keywords

Blotting, Southern; Chromatin/chemistry; Chromatin/genetics; Chromatin/metabolism; Cross-Linking Reagents/chemistry; DNA, Fungal/genetics; DNA, Fungal/metabolism; DNA, Ribosomal/genetics; DNA, Ribosomal/metabolism; Ficusin/chemistry; Gene Dosage; Genes, rRNA/genetics; High Mobility Group Proteins/genetics; High Mobility Group Proteins/metabolism; Histones/metabolism; Models, Genetic; Promoter Regions, Genetic/genetics; Protein Binding; Ribosomal Proteins/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:RRN10

enables

GO:0001165: RNA polymerase I cis-regulatory region sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:RRN7

enables

GO:0001164: RNA polymerase I core promoter sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:HMO1

located_in

GO:0033553: rDNA heterochromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:RRN11

enables

GO:0001164: RNA polymerase I core promoter sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:UAF30

enables

GO:0001165: RNA polymerase I cis-regulatory region sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:UAF30

enables

GO:0001165: RNA polymerase I upstream control element sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:HMO1

part_of

GO:0033553: rDNA heterochromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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