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PMID:18434412

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Citation

Benoit, P, Papin, C, Kwak, JE, Wickens, M and Simonelig, M (2008) PAP- and GLD-2-type poly(A) polymerases are required sequentially in cytoplasmic polyadenylation and oogenesis in Drosophila. Development 135:1969-79

Abstract

Cytoplasmic polyadenylation has an essential role in activating maternal mRNA translation during early development. In vertebrates, the reaction requires CPEB, an RNA-binding protein and the poly(A) polymerase GLD-2. GLD-2-type poly(A) polymerases form a family clearly distinguishable from canonical poly(A) polymerases (PAPs). In Drosophila, canonical PAP is involved in cytoplasmic polyadenylation with Orb, the Drosophila CPEB, during mid-oogenesis. We show that the female germline GLD-2 is encoded by wispy. Wispy acts as a poly(A) polymerase in a tethering assay and in vivo for cytoplasmic polyadenylation of specific mRNA targets during late oogenesis and early embryogenesis. wispy function is required at the final stage of oogenesis for metaphase of meiosis I arrest and for progression beyond this stage. By contrast, canonical PAP acts with Orb for the earliest steps of oogenesis. Both Wispy and PAP interact with Orb genetically and physically in an ovarian complex. We conclude that two distinct poly(A) polymerases have a role in cytoplasmic polyadenylation in the female germline, each of them being specifically required for different steps of oogenesis.

Links

PubMed Online version:10.1242/dev.021444

Keywords

Animals; Blotting, Western; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cytoplasm/metabolism; Drosophila/genetics; Drosophila/metabolism; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Eye Proteins/genetics; Eye Proteins/metabolism; Female; Gene Expression Regulation, Developmental; Immunoprecipitation; Meiosis/genetics; Metaphase/genetics; Oogenesis/genetics; Polyadenylation/genetics; Polynucleotide Adenylyltransferase/genetics; Polynucleotide Adenylyltransferase/metabolism; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:Q24433

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9VYS4

F

Seeded From UniProt

complete

DROME:GLD2B

involved_in

GO:0006378: mRNA polyadenylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:GLD2B

enables

GO:0004652: polynucleotide adenylyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:GLD2B

involved_in

GO:0043631: RNA polyadenylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:GLD2B

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q24433

F

Seeded From UniProt

complete

DROME:GLD2B

involved_in

GO:0048477: oogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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