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PMID:18404153

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Citation

Lacroix, M, El Messaoudi, S, Rodier, G, Le Cam, A, Sardet, C and Fabbrizio, E (2008) The histone-binding protein COPR5 is required for nuclear functions of the protein arginine methyltransferase PRMT5. EMBO Rep. 9:452-8

Abstract

Protein arginine methyltransferase 5 (PRMT5) targets nuclear and cytoplasmic proteins. Here, we identified a nuclear protein, called cooperator of PRMT5 (COPR5), involved in the nuclear functions of PRMT5. COPR5 tightly binds to PRMT5, both in vitro and in living cells, but not to other members of the PRMT family. PRMT5 bound to COPR5 methylates histone H4 (R3) preferentially when compared with histone H3 (R8), suggesting that COPR5 modulates the substrate specificity of nuclear PRMT5-containing complexes, at least towards histones. Markedly, recombinant COPR5 binds to the amino terminus of histone H4 and is required to recruit PRMT5 to reconstituted nucleosomes in vitro. Consistently, COPR5 depletion in cells strongly reduces PRMT5 recruitment on chromatin at the PRMT5 target gene cyclin E1 (CCNE1) in vivo. Moreover, both COPR5 depletion and overexpression affect CCNE1 promoter expression. We propose that COPR5 is an important chromatin adaptor for PRMT5 to function on a subset of its target genes.

Links

PubMed PMC2373370 Online version:10.1038/embor.2008.45

Keywords

Amino Acid Sequence; Arginine/metabolism; Carrier Proteins/chemistry; Carrier Proteins/genetics; Carrier Proteins/metabolism; Carrier Proteins/physiology; Cell Line, Tumor; Cell Nucleus/chemistry; Cell Nucleus/metabolism; Chromatin/metabolism; DNA Methylation; Genes, Reporter; Glutathione Transferase/metabolism; Histones/metabolism; Humans; Luciferases/metabolism; Molecular Sequence Data; Nuclear Proteins/metabolism; Nuclear Proteins/physiology; Protein Binding; Protein Methyltransferases/metabolism; Protein Methyltransferases/physiology; Protein Structure, Secondary; Recombinant Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:ANM5

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9NQ92

F

Seeded From UniProt

complete

HUMAN:ANM5

located_in

GO:0005634: nucleus

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

HUMAN:H4

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9NQ92

F

Seeded From UniProt

complete

HUMAN:COPRS

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O14744

F

Seeded From UniProt

complete

HUMAN:COPRS

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:COPRS

enables

GO:0042393: histone binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:COPRS

involved_in

GO:0043985: histone H4-R3 methylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:COPRS

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P62805

F

Seeded From UniProt

complete

HUMAN:ANM5

part_of

GO:0005634: nucleus

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

HUMAN:ANM5

involved_in

GO:0043985: histone H4-R3 methylation

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete


See also

References

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